Mucinous secretion from canine Heidenhain pouch after stimulation with food, pentagastrin and histamine.

Principal glycoproteins and amino acids were determined in gastric juice obtained from Heidenhain pouches of Labrador pure-bred dogs. Secretions were stimulated by food, pentagastrin or histamine. Non-stimulated fasting secretions served as controls. All samples showed the presence of galactose, glucosamine, galactosamine, fucose, glucose, mannose and sulfate. Galactose and glucosamine were found in equimolar amount and the galactose to galactosamine ratio was 2:1. Concentrations of most carbohydrate components studied were higher in fasting than in stimulated secretions. Outputs of some of these components were lower in fasting than in stimulated secretions. Modes of stimulation used did not significantly affect the outputs of principal glycoproteins studied. Compared with human gastric secretions studied by others, using similar methods, certain differences were found particularly in the relationship between some carbohydrate components and between amino acids.