Uren M F, Walker P J, Zakrzewski H, St George T D, Byrne K A
CSIRO Division of Tropical Animal Production, Long Pocket Laboratories, Indooroopilly, Queensland, Australia.
Vaccine. 1994 Jul;12(9):845-50. doi: 10.1016/0264-410x(94)90295-x.
In a series of experiments, the envelope glycoprotein (G protein) of bovine ephemeral fever virus (BEFV) induced immunity against challenge with virulent virus. Protection correlated with the level of specific serum antibodies to G protein measured by a blocking ELISA test and with the level of neutralizing antibody. The optimum vaccination regimen consisted of two injections given 21 days apart at a dose rate of 0.32 microgram per cow of purified G protein emulsified in the adjuvant Quil A. This schedule conferred immunity for the duration of the preliminary experiment (46 days). Immunity to severe disease, but not to infection, remained for at least 12 months after vaccination, although BEFV could not be reisolated from vaccinated cattle following challenge. Unvaccinated cattle used as controls exhibited typical signs of clinical ephemeral fever and BEFV was recovered from all control animals following challenge.
在一系列实验中，牛暂时热病毒（BEFV）的包膜糖蛋白（G蛋白）可诱导机体产生针对强毒病毒攻击的免疫力。保护作用与通过阻断ELISA试验测得的针对G蛋白的特异性血清抗体水平以及中和抗体水平相关。最佳疫苗接种方案为以每头牛0.32微克纯化G蛋白的剂量率，在佐剂Quil A中乳化，每隔21天注射两次。该方案在初步实验期间（46天）赋予了免疫力。接种疫苗后，对严重疾病的免疫力（而非对感染的免疫力）至少持续12个月，尽管在攻击后无法从接种疫苗的牛中重新分离出BEFV。用作对照的未接种疫苗的牛表现出典型的临床暂时热症状，并且在攻击后从所有对照动物中均分离出了BEFV。
