Domeika M A, Bassiri M, Mårdh P A
Institute of Clinical Bacteriology, University of Uppsala, Sweden.
Acta Derm Venereol. 1994 Jul;74(4):323-6. doi: 10.2340/0001555574323326.
First-void urine specimens, collected from 309 military recruits, 246 male adolescent gymnasium students and 194 patients consulting venereal disease clinics, were studied for the presence of Chlamydia trachomatis with the use of antigen detection tests--two enzyme immunoassays (EIA) and a direct immunofluorescence test (DIF; Syva MicroTrak). Urethral swabs were collected when discrepancies between the EIA and DIF tests were detected. The patient was regarded as positive when the culture result was positive or when two antigen detection tests corraborated one another. The Syva MicroTrak EIA and DIF tests were more sensitive than the Orion EIA, i.e. 98.5%, 99.2% and 74%, respectively. This was true when testing both low- and high-risk groups, with a prevalence of chlamydial infection ranging from 0.4% to 58.6%. All three tests were highly specific. The positive predictive values for the Syva MicroTrak EIA, the DIF and the Orion EIA were 99.2%, 100% and 100%, respectively and the negative predictive values 99.8%, 99.8% and 94.8%, respectively.
收集了309名新兵、246名男性青少年体育馆学生和194名性病门诊患者的首次晨尿标本，使用抗原检测试验——两种酶免疫测定法（EIA）和一种直接免疫荧光试验（DIF；Syva MicroTrak）研究沙眼衣原体的存在情况。当检测到EIA和DIF试验结果不一致时，收集尿道拭子。当培养结果为阳性或两种抗原检测试验结果相互证实时，患者被视为阳性。Syva MicroTrak EIA和DIF试验比Orion EIA更敏感，分别为98.5%、99.2%和74%。在检测低风险和高风险组时都是如此，衣原体感染率在0.4%至58.6%之间。所有三种试验都具有高度特异性。Syva MicroTrak EIA、DIF和Orion EIA的阳性预测值分别为99.2%、100%和100%，阴性预测值分别为99.8%、99.8%和94.8%。
