Moav N, Steinberg L, Frensdorff A
Int Arch Allergy Appl Immunol. 1976;52(1-4):257-65. doi: 10.1159/000231690.
The effect of vinblastine (VLB), a mitotic blocking agent, on the number of plaque-forming cells (PFC) and on the metabolic activities of spleen cells of mice reimmunized with SRBC was studied. When VLB (75 mug/mouse) and antigen were administered simultaneously, the number of pfc, on the 4th day after immunization, was reduced to 40% of control levels. However, when the same amount of VLB was administered to mice 24 h after immunization, it reduced the number of PFC to 10% of control levels. The possibility that VLB exerts a specific cytotoxic effect on preformed PFC either in vivo or in vitro was ruled out. A direct profound effect of VLB on antigen-stimulated cells was observed when VLB was injected 24 h after reimmunization, and the incorporation rates of radioactive precursors into macromolecules by spleen cells were measured at 4-hour intervals. VLB suppressed completely the antigen-induced peak of 3H-thymiding incorporation, while it had no effect on the incorporation rate of 3H-uridine and only a slight effect on the incorporation rate of 3H-amino-acids by the same cells. The results suggest that the decrease in number of PFC caused by injection of VLB 24 h after immunization is due to prevention by VLB, of precursor cells from going through a critical cell division, which takes place later than 24 h after immunization. Thus, at least one cell division is required for an immune response in vivo.
研究了有丝分裂阻断剂长春花碱（VLB）对再次用绵羊红细胞（SRBC）免疫的小鼠的空斑形成细胞（PFC）数量及脾细胞代谢活性的影响。当同时给予VLB（75微克/只小鼠）和抗原时，免疫后第4天的PFC数量降至对照水平的40%。然而，当在免疫后24小时给小鼠注射相同剂量的VLB时，PFC数量降至对照水平的10%。排除了VLB在体内或体外对预先形成的PFC产生特异性细胞毒性作用的可能性。再次免疫后24小时注射VLB时，观察到VLB对抗原刺激细胞有直接的显著作用，并每隔4小时测量脾细胞将放射性前体掺入大分子的掺入率。VLB完全抑制了抗原诱导的3H-胸腺嘧啶掺入峰值，而对相同细胞的3H-尿苷掺入率没有影响，对3H-氨基酸掺入率只有轻微影响。结果表明，免疫后24小时注射VLB导致PFC数量减少是由于VLB阻止了前体细胞经历关键的细胞分裂，这种细胞分裂发生在免疫后24小时之后。因此，体内免疫反应至少需要一次细胞分裂。
