Cohen N D, McGruder E D, Neibergs H L, Behle R W, Wallis D E, Hargis B M
Department of Large Animal Medicine and Surgery, College of Veterinary Medicine, Texas A&M University, College Station 77843.
Poult Sci. 1994 Feb;73(2):354-7. doi: 10.3382/ps.0730354.
Salmonella enteritidis was identified in feces from hens using the polymerase chain reaction (PCR) and oligonucleotide primers specific for all members of the genus Salmonella. Feces from specific-pathogen-free Leghorn hens were determined to be negative for Salmonella by microbiological culture and by the PCR. Fecal samples were inoculated with known numbers of colony-forming units of S. enteritidis. The DNA was extracted from fecal samples and amplified by the PCR using genus-specific primers. Salmonella were detected in all samples known to be positive; the sensitivity of the assay extended to 1 cfu of S. enteritidis/g feces. Feces that were not inoculated with Salmonella were negative. Microbiological culture was less sensitive than the PCR assay; results of culture of feces with less than 10(2) cfu/g were negative. Although S. enteritidis was used in this study, the oligonucleotide primers used in this study have been previously demonstrated to be genus-specific for Salmonella.
利用聚合酶链反应（PCR）和对沙门氏菌属所有成员具有特异性的寡核苷酸引物，在母鸡粪便中鉴定出肠炎沙门氏菌。通过微生物培养和PCR检测，确定无特定病原体的来航鸡粪便中沙门氏菌呈阴性。用已知数量的肠炎沙门氏菌菌落形成单位接种粪便样本。从粪便样本中提取DNA，并使用属特异性引物通过PCR进行扩增。在所有已知为阳性的样本中均检测到沙门氏菌；该检测方法的灵敏度可延伸至每克粪便中1个肠炎沙门氏菌菌落形成单位。未接种沙门氏菌的粪便呈阴性。微生物培养比PCR检测灵敏度低；每克粪便中菌落形成单位少于10²的粪便培养结果为阴性。尽管本研究中使用了肠炎沙门氏菌，但本研究中使用的寡核苷酸引物此前已被证明对沙门氏菌属具有特异性。
