一名死于先天性完全性心脏传导阻滞儿童心脏洗脱液中针对天然60-kd Ro/SS-A和变性52-kd Ro/SS-A自身抗体的浓度。
Concentration of autoantibodies to native 60-kd Ro/SS-A and denatured 52-kd Ro/SS-A in eluates from the heart of a child who died with congenital complete heart block.
作者信息
Reichlin M, Brucato A, Frank M B, Maddison P J, McCubbin V R, Wolfson-Reichlin M, Lee L A
机构信息
Oklahoma Medical Research Foundation, Oklahoma City 73104.
出版信息
Arthritis Rheum. 1994 Nov;37(11):1698-703. doi: 10.1002/art.1780371120.
OBJECTIVE
To determine the serologic specificity of acid eluates from tissues of a child who died with congenital complete heart block (CCHB).
METHODS
Tissues were extracted, acid eluted, and the IgG and antibody titers determined on the eluates by enzyme-linked immunosorbent assay.
RESULTS
Antibodies to native 60-kd and denatured 52-kd Ro/SS-A were found to be enriched only in the heart eluate, and not in the eluates from brain, kidney, and skin.
CONCLUSION
These findings indicate a major role for anti-native 60-kd Ro/SS-A in the immunopathogenesis of CCHB.
目的
确定一名死于先天性完全性心脏传导阻滞（CCHB）儿童组织中酸洗脱液的血清学特异性。
方法
提取组织，进行酸洗脱，并通过酶联免疫吸附测定法测定洗脱液中的IgG和抗体滴度。
结果
发现针对天然60-kd和变性52-kd Ro/SS-A的抗体仅在心脏洗脱液中富集，而在脑、肾和皮肤的洗脱液中未富集。
结论
这些发现表明抗天然60-kd Ro/SS-A在CCHB的免疫发病机制中起主要作用。
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