Chuah S Y, Tan W F, Yap K H, Tai H E, Chow S T
Institute of Science and Forensic Medicine, Singapore.
Forensic Sci Int. 1994 Oct 21;68(3):169-80. doi: 10.1016/0379-0738(94)90356-5.
The distributions of the D1S80 alleles and genotypes in the Chinese, Malays and Indians in Singapore were determined by amplified fragment length polymorphism (AMP-FLP) analysis. The distributions of the observed genotypes for the three races conformed to Hardy-Weinberg expectations. The system was applied to 19 families whose paternity had been established by restriction fragment length polymorphism (RFLP) analysis. In all cases, Mendelian inheritance of the alleles at the D1S80 locus could be demonstrated. D1S80 typing on DNA recovered by differential extraction of forensic specimens which included vaginal swabs, urethral swabs and seminal stains yielded consistent results.
通过扩增片段长度多态性（AMP-FLP）分析确定了新加坡华人、马来人和印度人中D1S80等位基因和基因型的分布。三个种族观察到的基因型分布符合哈迪-温伯格预期。该系统应用于19个通过限制性片段长度多态性（RFLP）分析确定了父子关系的家庭。在所有情况下，都可以证明D1S80位点等位基因的孟德尔遗传。对包括阴道拭子、尿道拭子和精液斑在内的法医标本进行差异提取后回收的DNA进行D1S80分型，结果一致。
