Puvion-Dutilleul F, Leduc E H, Puvion E
Laboratoire Organisation fonctionnelle du Noyau, Institut de Recherches sur le Cancer/IFC1, UPR 9044 CNRS, Villejuif, France.
Biol Cell. 1996;87(3):133-41.
The Feulgen-like osmium ammine-SO2 method developed by Cogliati and Gautier (CR Acad Sci Ser D 1973, 276, 3041-3044) stains the DNA at the ultrastructural level. Compared to several other techniques for detecting DNA, this method remains the only one revealing the configuration of the DNA molecules within the cell whatever their compactness. In the present article we summarize the results we obtained with the osmium ammine method in the study of the fate of viral genomes along the infectious cycles in several DNA virus infected cells including adenovirus, herpes simplex virus, simian virus 40 and poxvirus. The results are discussed in relation to the replicative and transcribing activities of viral DNA.
由科利亚蒂和 Gautier 开发的（《法国科学院院报》D 辑，1973 年，276 卷，3041 - 3044 页）类福尔根锇胺 - 二氧化硫方法可在超微结构水平对 DNA 进行染色。与其他几种检测 DNA 的技术相比，无论 DNA 分子的紧密程度如何，该方法仍是唯一能揭示细胞内 DNA 分子构型的方法。在本文中，我们总结了在研究包括腺病毒、单纯疱疹病毒、猴病毒 40 和痘病毒在内的几种 DNA 病毒感染细胞中病毒基因组在感染周期中的命运时，使用锇胺方法所获得的结果。并结合病毒 DNA 的复制和转录活性对结果进行了讨论。
