人类免疫缺陷病毒1型（HIV-1）gp120高变区1和高变区2之间高度保守的天冬氨酸残基对病毒复制早期阶段很重要。
The highly conserved aspartic acid residue between hypervariable regions 1 and 2 of human immunodeficiency virus type 1 gp120 is important for early stages of virus replication.
作者信息
Wang W K, Essex M, Lee T H
机构信息
Department of Cancer Biology, Harvard School of Public Health, Boston, Massachusetts 02115.
出版信息
J Virol. 1995 Jan;69(1):538-42. doi: 10.1128/JVI.69.1.538-542.1995.
Abstract
Between hypervariable regions V1 and V2 of human immunodeficiency virus type 1 (HIV-1) gp120 lies a cluster of relatively conserved residues. The contribution of nine charged residues in this region to virus infectivity was evaluated by single-amino-acid substitutions in an infectious provirus clone. Three of the HIV-1 mutants studied had slower growth kinetics than the wild-type virus. The delay was most pronounced in a mutant with an alanine substituted for an aspartic acid residue at position 180. This aspartic acid is conserved by all HIV-1 isolates with known nucleotide sequences. Substitutions with three other residues at this position, including a negatively charged glutamic acid, all affected virus infectivity. The defect identified in these mutants suggests that this aspartic acid residue is involved in the early stages of HIV-1 replication.
摘要
在人类免疫缺陷病毒1型（HIV-1）gp120的高变区V1和V2之间存在一组相对保守的残基。通过在感染性前病毒克隆中进行单氨基酸替换，评估了该区域九个带电荷残基对病毒感染性的作用。所研究的三个HIV-1突变体的生长动力学比野生型病毒慢。这种延迟在一个将丙氨酸替换第180位天冬氨酸残基的突变体中最为明显。该天冬氨酸在所有已知核苷酸序列的HIV-1分离株中都是保守的。在该位置用其他三个残基进行替换，包括带负电荷的谷氨酸，均影响病毒感染性。在这些突变体中发现的缺陷表明，该天冬氨酸残基参与了HIV-1复制的早期阶段。
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