Heydari A R, Takahashi R, Gutsmann A, You S, Richardson A
Geriatric Research, Education and Clinical Center, Audie L., Murphy Memorial Veterans Hospital, San Antonio, Texas.
Experientia. 1994 Nov 30;50(11-12):1092-8. doi: 10.1007/BF01923466.
An alteration in the ability of cells to express heat shock proteins could be physiologically important in aging because all living organisms show a reduced ability to respond to stress with increasing age. Using hepatocytes freshly isolated from young adult and old rats, we have shown that the induction of hsp70 expression by heat shock is reduced approximately 50% with age. The decrease in hsp70 expression occurs at the level of transcription and appears to arise from a defect in the heat shock transcription factor. Other investigators have also shown that the induction of hsp70 expression by heat shock as well as other stresses declines significantly with age in a variety of tissues from rats as well as mononuclear cells from human subjects. In addition, a decrease in the inducibility of hsp70 is observed with cell senescence in cultured cells. Therefore, it appears that a reduced ability to express hsp70 in response to stress may be a common phenomenon underlying the aging process.
细胞表达热休克蛋白能力的改变在衰老过程中可能具有重要的生理意义，因为所有生物随着年龄增长对压力的反应能力都会下降。我们使用从年轻成年大鼠和老年大鼠新鲜分离的肝细胞，发现热休克诱导的hsp70表达随年龄增长降低了约50%。hsp70表达的降低发生在转录水平，似乎是由热休克转录因子的缺陷引起的。其他研究人员也表明，热休克以及其他应激诱导的hsp70表达在大鼠的各种组织以及人类受试者的单核细胞中均随年龄显著下降。此外，在培养细胞中，随着细胞衰老，hsp70的诱导性也会降低。因此，似乎应激时表达hsp70的能力降低可能是衰老过程的一个普遍现象。
