Stoakes L, John M A, Lannigan R, Schieven B C, Ramos M, Harley D, Hussain Z
Department of Clinical Microbiology, Victoria Hospital, London, Ontario, Canada.
J Clin Microbiol. 1994 Aug;32(8):1908-10. doi: 10.1128/jcm.32.8.1908-1910.1994.
A commercially available, computer-assisted microbial identification system (MIS) employs gas-liquid chromatographic analyses of bacterial fatty acids. The MIS was used to identify 470 isolates of Staphylococcus species. The accuracy of the MIS was compared with the accuracies of conventional methods. There was a complete agreement between the MIS and conventional methods in the identification of 413 (87.8%) strains. For 36 of 45 misidentified strains, the correct identification was listed by the MIS as a choice but not as the first choice. Twelve strains could not be matched. All strains of Staphylococcus cohnii, S. epidermidis, S. intermedius, S. lugdunensis, S. schleiferi, S. sciuri, S. simulans, and S. xylosus were correctly identified. Two species, S. hominis and S. saprophyticus, accounted for 52.6% (30 of 57) of the misidentifications. Seventy-eight organisms were retested. Identification of 73 organisms remained unchanged, and for five organisms, the second choice became first and vice versa. The overall performance of the MIS is acceptable, and the system can be used as an alternate identification method for staphylococci.
一种市售的计算机辅助微生物鉴定系统（MIS）采用细菌脂肪酸的气-液色谱分析。该MIS用于鉴定470株葡萄球菌属菌株。将MIS的准确性与传统方法的准确性进行了比较。在413株（87.8%）菌株的鉴定中，MIS与传统方法完全一致。在45株误鉴定的菌株中，有36株的正确鉴定在MIS中被列为一个选项，但不是首选。有12株无法匹配。所有科氏葡萄球菌、表皮葡萄球菌、中间葡萄球菌、路邓葡萄球菌、施氏葡萄球菌、松鼠葡萄球菌、模仿葡萄球菌和木糖葡萄球菌菌株均被正确鉴定。人葡萄球菌和腐生葡萄球菌这两个菌种占误鉴定的52.6%（57株中的30株）。对78个菌株进行了重新检测。73个菌株的鉴定结果保持不变，对于5个菌株，原来的第二选择变成了第一选择，反之亦然。MIS的总体性能是可以接受的，该系统可作为葡萄球菌的一种替代鉴定方法。
