使用MicroScan Pos ID和快速Pos ID鉴定系统鉴定葡萄球菌属菌种及亚种。
Identification of Staphylococcus species and subspecies with the MicroScan Pos ID and Rapid Pos ID panel systems.
作者信息
Kloos W E, George C G
机构信息
Department of Genetics, North Carolina State University, Raleigh 27695-7614.
出版信息
J Clin Microbiol. 1991 Apr;29(4):738-44. doi: 10.1128/jcm.29.4.738-744.1991.
The accuracies of the MicroScan Pos ID and Rapid Pos ID panel systems (Baxter Diagnostic Inc., MicroScan Division, West Sacramento, Calif.) were compared with each other and with the accuracies of conventional methods for the identification of 25 Staphylococcus species and 4 subspecies. Conventional methods included those used in the original descriptions of species and subspecies and DNA-DNA hybridization. The Pos ID panel uses a battery of 18 tests, and the Rapid Pos ID panel uses a battery of 42 tests for the identification of Staphylococcus species. The Pos ID panel has modified conventional and chromogenic tests that can be read after 15 to 48 h of incubation; the Rapid Pos ID panel has tests that use fluorogenic substrates or fluorometric indicators, and test results can be read after 2 h of incubation in the autoSCAN-W/A. Results indicated that both MicroScan systems had a high degree of congruence (greater than or equal to 90%) with conventional methods for the species S. capitis, S. aureus, S. auricularis, S. saprophyticus, S. cohnii, S. arlettae, S. carnosus, S. lentus, and S. sciuri and, in particular, the subspecies S. capitis subsp. capitis and S. cohnii subsp. cohnii. The Rapid Pos ID panel system also had greater than or equal to 90% congruence with conventional methods for S. epidermidis, S. caprae, S. warneri subsp. 2, S. xylosus, S. kloosii, and S. caseolyticus. For both MicroScan systems, congruence with conventional methods was 80 to 90% for S. haemolyticus subsp. 1, S. equorum, S. intermedius, and S. hyicus; and in addition, with the Rapid Pos ID panel system congruence was 80 to 89% for S. capitis subsp. ureolyticus, S. warneri subsp. 1, S. hominis, S. cohnii subsp. urealyticum, and S. simulans. The MicroScan systems identified a lower percentage (50 to 75%) of strains of S. lugdunensis, S. gallinarum, S. schleiferi, and S. chromogenes, although the addition of specific tests to the systems might increase the accuracy of identification significantly.
将MicroScan Pos ID和Rapid Pos ID鉴定板系统（百特诊断公司，MicroScan部门，加利福尼亚州西萨克拉门托）的准确性相互进行了比较，并与用于鉴定25种葡萄球菌和4个亚种的传统方法的准确性进行了比较。传统方法包括物种和亚种原始描述中使用的方法以及DNA - DNA杂交。Pos ID鉴定板使用一组18项测试，Rapid Pos ID鉴定板使用一组42项测试来鉴定葡萄球菌。Pos ID鉴定板对传统测试和显色测试进行了改进，可在孵育15至48小时后读取结果；Rapid Pos ID鉴定板的测试使用荧光底物或荧光指示剂，在autoSCAN - W/A中孵育2小时后即可读取测试结果。结果表明，对于头状葡萄球菌、金黄色葡萄球菌、耳葡萄球菌、腐生葡萄球菌、科氏葡萄球菌、阿氏葡萄球菌、肉葡萄球菌、迟缓葡萄球菌和松鼠葡萄球菌，特别是头状葡萄球菌头状亚种和科氏葡萄球菌科氏亚种，两种MicroScan系统与传统方法的一致性都很高（大于或等于90%）。对于表皮葡萄球菌、山羊葡萄球菌、华纳葡萄球菌2亚种、木糖葡萄球菌、克氏葡萄球菌和解脲葡萄球菌，Rapid Pos ID鉴定板系统与传统方法的一致性也大于或等于90%。对于两种MicroScan系统，溶血葡萄球菌1亚种、马胃葡萄球菌、中间葡萄球菌和猪葡萄球菌与传统方法的一致性为80%至90%；此外，对于解脲头状葡萄球菌、华纳葡萄球菌1亚种、人葡萄球菌、解脲科氏葡萄球菌和模仿葡萄球菌，Rapid Pos ID鉴定板系统与传统方法的一致性为80%至89%。MicroScan系统鉴定出的路邓葡萄球菌、鸡葡萄球菌、施氏葡萄球菌和产色葡萄球菌菌株的比例较低（50%至75%），不过在系统中添加特定测试可能会显著提高鉴定的准确性。
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