两种用于凝固酶阴性葡萄球菌种水平鉴定的商业系统的评估。
Evaluation of two commercial systems for identification of coagulase-negative staphylococci to species level.
作者信息
Grant C E, Sewell D L, Pfaller M, Bumgardner R V, Williams J A
机构信息
Department of Pathology, Oregon Health Sciences University, Portland.
出版信息
Diagn Microbiol Infect Dis. 1994 Jan;18(1):1-5. doi: 10.1016/0732-8893(94)90126-0.
Bloodstream (224) and urine (nine) isolates of coagulase-negative staphylococci (CNS) were evaluated using MicroScan Pos ID and Rapid Pos ID panels. A modification of the conventional method of Kloos and Schleifer served as the reference method. The isolates were selected to include a broad range of CNS species, including 44 S. epidermidis, 50 S. hominis, 39 S. warneri, 33 S. capitis, 21 S. haemolyticus, 12 S. simulans, 11 S. saprophyticus, six S. cohnii, five S. lugdunensis, three S. xylosus, four S. auricularis, two S. schleiferi, two S. intermedius, and one S. sciuri. The Pos ID panel had an overall rate of agreement (correct plus probably correct) with the reference method of 79%, including 95% for S. epidermidis, 95% for S. haemolyticus, 64% for S. hominis, 67% for S. simulans, 79% for S. warneri, and 100% for S. saprophyticus. The Rapid Pos ID panel had an overall rate of agreement (correct plus probably correct) of 76%, including 91% for S. epidermidis, 90% for S. haemolyticus, 64% for S. hominis, 58% for S. simulans, 77% for S. warneri, and 100% for S. saprophyticus. Both systems are acceptable for the identification of the clinically significant species S. haemolyticus, S. saprophyticus, and S. epidermidis, but are less reliable for the infrequently isolated species of CNS.
使用MicroScan Pos ID和Rapid Pos ID鉴定板对凝固酶阴性葡萄球菌（CNS）的血流分离株（224株）和尿液分离株（9株）进行了评估。对Kloos和Schleifer的传统方法进行改良后作为参考方法。所选择的分离株涵盖了广泛的CNS菌种，包括44株表皮葡萄球菌、50株人葡萄球菌、39株华纳葡萄球菌、33株头葡萄球菌、21株溶血葡萄球菌、12株模仿葡萄球菌、11株腐生葡萄球菌、6株科氏葡萄球菌、5株路邓葡萄球菌、3株木糖葡萄球菌、4株耳葡萄球菌、2株施氏葡萄球菌、2株中间葡萄球菌和1株松鼠葡萄球菌。Pos ID鉴定板与参考方法的总体符合率（正确加可能正确）为79%，其中表皮葡萄球菌为95%，溶血葡萄球菌为95%，人葡萄球菌为64%，模仿葡萄球菌为67%，华纳葡萄球菌为79%，腐生葡萄球菌为100%。Rapid Pos ID鉴定板的总体符合率（正确加可能正确）为76%，其中表皮葡萄球菌为91%，溶血葡萄球菌为90%，人葡萄球菌为64%，模仿葡萄球菌为58%，华纳葡萄球菌为77%，腐生葡萄球菌为100%。这两种系统对于鉴定具有临床意义的溶血葡萄球菌、腐生葡萄球菌和表皮葡萄球菌是可接受的，但对于CNS中较少分离的菌种可靠性较低。
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