Guth L, Zhang Z, Roberts E
Department of Biology, College of William and Mary, Williamsburg, VA 23187.
Proc Natl Acad Sci U S A. 1994 Dec 6;91(25):12308-12. doi: 10.1073/pnas.91.25.12308.
Controlled compressive injury to rat spinal cord was chosen to test therapies that might attenuate the progression of tissue destruction and locomotor deficits that characteristically occur after spinal injury. A highly significant reduction of damage was achieved by immediate postinjury treatment with a combination of the following: an antiinflammatory substance, indomethacin; a stimulator of cytokine secretion, bacterial lipopolysaccharide; and the parent steroid, from which all other steroids arise, pregnenolone. This treatment reduced histopathological changes, spared tissue from secondary injury, and increased restoration of motor function. Remarkably, 11 of 16 of the animals treated with the above combination were able to stand and walk at 21 days after injury, 4 of them almost normally. The results were far superior to those obtained in controls or in animals to which the substances were given separately or in combination of two. This approach may prove to be applicable to nervous system injury, in general, and to injury in other tissues.
选择对大鼠脊髓进行控制性压迫损伤，以测试可能减轻脊髓损伤后典型发生的组织破坏进展和运动功能障碍的治疗方法。通过在损伤后立即联合使用以下物质进行治疗，损伤得到了显著减轻：一种抗炎物质吲哚美辛；一种细胞因子分泌刺激剂细菌脂多糖；以及所有其他类固醇的母体类固醇孕烯醇酮。这种治疗减少了组织病理学变化，使组织免受继发性损伤，并增强了运动功能的恢复。值得注意的是，用上述组合治疗的16只动物中有11只在损伤后21天能够站立和行走，其中4只几乎正常行走。结果远优于对照组或单独给予这些物质或两两组合给予动物所获得的结果。这种方法可能证明一般适用于神经系统损伤以及其他组织的损伤。
