Nakase T, Takaoka K, Hirakawa K, Hirota S, Takemura T, Onoue H, Takebayashi K, Kitamura Y, Nomura S
Department of Orthopaedic Surgery, Osaka University Medical School, Suita, Japan.
Bone Miner. 1994 Aug;26(2):109-22. doi: 10.1016/s0169-6009(08)80056-6.
Heterogeneity in the expression of three members of non-collagenous matrix proteins in osteogenic and chondrogenic development in vivo were investigated by in situ hybridization. Sections of several skeletal tissues from mice at various stages of development were hybridized with digoxigenin-labeled complementary RNA probes encoding osteonectin (Osn), osteopontin (Osp) and osteocalcin (Osc). In calvariae and mandibulae, Osn messenger RNA (mRNA) was detected in cells in pre-osseous and osseous tissues before mineralization. Osp mRNA was found in cells attached to the mineralized bone matrix together with Osn mRNA followed by the expression Osc mRNA. In long bones, mRNAs for Osn, Osp and Osc were sequentially expressed with bone development from primary spongiosa to diaphyseal bone. In growth cartilage, Osn mRNA was observed in chondrocytes in non-mineralized cartilage, whereas Osp mRNA was detected in hypertrophic chondrocytes in mineralized cartilage matrix with a characteristic switch in expression. Osc mRNA was not detected in any chondrocytes. These results indicate that osteogenic differentiation in bone development in vivo is characterized by the sequential expression of these three genes, and suggest that these genes are expressed differentially and specifically, in association with extra-cellular matrix mineralization.
通过原位杂交研究了体内成骨和软骨形成发育过程中三种非胶原蛋白基质蛋白成员表达的异质性。用编码骨连接蛋白（Osn）、骨桥蛋白（Osp）和骨钙素（Osc）的地高辛标记互补RNA探针与处于不同发育阶段的小鼠的几种骨骼组织切片进行杂交。在颅骨和下颌骨中，在矿化前的骨前组织和骨组织中的细胞中检测到Osn信使核糖核酸（mRNA）。Osp mRNA与Osn mRNA一起在附着于矿化骨基质的细胞中被发现，随后表达Osc mRNA。在长骨中，随着骨从初级骨松质向骨干骨发育，Osn、Osp和Osc的mRNA依次表达。在生长软骨中，在未矿化软骨的软骨细胞中观察到Osn mRNA，而在矿化软骨基质中的肥大软骨细胞中检测到Osp mRNA，其表达有特征性转变。在任何软骨细胞中均未检测到Osc mRNA。这些结果表明，体内骨发育中的成骨分化以这三个基因的顺序表达为特征，并表明这些基因与细胞外基质矿化相关，表达存在差异且具有特异性。
