Polimeno L, Silecchia G, Spaziani E, Scucchi A, Dell'Aquila P, Ierardi E, Materia A, Giangaspero A, Basso N, Francavilla A
Department of Gastroenterology, University of Bari, Italy.
Dig Dis Sci. 1994 Mar;39(3):635-40. doi: 10.1007/BF02088353.
Complex and conflicting relationships between epidermal growth factor (EGF), estrogens (E), androgens (A), and related receptors (EGF-R, E-R, A-R) have been reported in different biological situations associated with cell proliferation. There is also evidence that EGF and sex hormone receptors may be involved in normal and neoplastic growth of the gastrointestinal mucosa. In this study, we investigated the behavior of EGF receptors and sex hormone and related receptors, during N-methyl-N'-nitro-N-nitrosoguanidine (NG)-induced gastric carcinogenesis in Sprague-Dawley male rats. Four groups of 15 rats each (10 NG-treated and five controls) were sacrificed after 1, 20, 30, and 40 weeks of treatment. Gastric tissue from each rat was processed for receptor status (number and affinity) and proliferative activity. A significant and progressive decrease of A-R and EGF-R was observed starting from the 20th week, while no change of E-R occurred throughout the experiment. Cell proliferation in the gastric mucosa of NG-treated rats increased after 30 weeks of treatment. These data indicate that NG treatment is able to modify the receptor status of gastric mucosa in rats.
在与细胞增殖相关的不同生物学情况下，已报道了表皮生长因子（EGF）、雌激素（E）、雄激素（A）及相关受体（EGF-R、E-R、A-R）之间复杂且相互矛盾的关系。也有证据表明，EGF和性激素受体可能参与胃肠道黏膜的正常生长和肿瘤生长。在本研究中，我们调查了在N-甲基-N'-硝基-N-亚硝基胍（NG）诱导的Sprague-Dawley雄性大鼠胃癌发生过程中，EGF受体、性激素及相关受体的行为。每组15只大鼠（10只经NG处理，5只为对照）在处理1、20、30和40周后处死。对每只大鼠的胃组织进行受体状态（数量和亲和力）及增殖活性检测。从第20周开始观察到A-R和EGF-R显著且逐渐降低，而在整个实验过程中E-R未发生变化。经NG处理的大鼠胃黏膜细胞增殖在处理30周后增加。这些数据表明，NG处理能够改变大鼠胃黏膜的受体状态。
