Safayhi H, Sabieraj J, Sailer E R, Ammon H P
Department of Pharmacology, University of Tübingen, Federal Republic of Germany.
Planta Med. 1994 Oct;60(5):410-3. doi: 10.1055/s-2006-959520.
Matricine and its transformation product chamazulene are constituents of chamomile extracts. Both have been demonstrated to exert anti-inflammatory activity in vivo. Since preparations from chamomile are used for the treatment of inflammatory skin and bowel diseases, we studied the effects of these compounds on the leukotriene production in neutrophilic granulocytes. Chamazulene inhibited the formation of leukotriene B4 in intact cells and in the 105,000 x g supernatant fraction in a concentration-dependent manner. The IC50 values were 15 and 10 microM, respectively. Matricine showed no effect up to 200 microM. Chamazulene (IC50: 2 microM), but not matricine, blocked the chemical peroxidation of arachidonic acid. Additionally, matricine (up to 200 microM) had no effects on the cyclooxygenase and 12-lipoxygenase activities in human platelets. Therefore, it is concluded that chamazulene, but not matricine, may contribute to the anti-inflammatory activity of chamomile extracts by inhibiting the leukotriene synthesis and additional antioxidative effects.
母菊天蓝烃及其转化产物天蓝烃是洋甘菊提取物的成分。二者均已被证明在体内具有抗炎活性。由于洋甘菊制剂用于治疗炎性皮肤病和肠道疾病，我们研究了这些化合物对嗜中性粒细胞中白三烯生成的影响。天蓝烃以浓度依赖的方式抑制完整细胞和105,000×g上清液组分中白三烯B4的形成。IC50值分别为15和10 microM。母菊天蓝烃在高达200 microM时无作用。天蓝烃（IC50：2 microM）而非母菊天蓝烃可阻断花生四烯酸的化学过氧化反应。此外，母菊天蓝烃（高达200 microM）对人血小板中的环氧化酶和12-脂氧合酶活性无影响。因此，可以得出结论，天蓝烃而非母菊天蓝烃可能通过抑制白三烯合成和其他抗氧化作用而有助于洋甘菊提取物的抗炎活性。
