Timenetsky J, Curcio M
Departamento de Microbiologia, Universidade de São Paulo, Brasil.
Rev Inst Med Trop Sao Paulo. 1993 Nov-Dec;35(6):551-5.
Staphylococcal Coagglutination was used as method for a rapid identification of mycoplasmas that could be performed by non specialized laboratories. Suspensions of Staphylococcus aureus (Cowan I) sensitized with rabbit antibodies against NCTC mycoplasma strains have identified these microorganisms and the strains isolated from humans, cell cultures rats and mice in concentrated suspensions from cultures of 4.0 ml. Fourty eight strains of M.pulmonis, 6 of M. arthritidis, 8 of M.arginini, 3 of M.orale, 15 of A.laidlawii, 8 of M.hominis and 3 of M.pneumoniae were identified by staphylococcal coagglutination and confirmed by Growth Inhibition Test. Optimal parameters of coagglutination were established and the stability of the conjugates were preserved for 90 days when added with acetyl cysteine. The reaction was visualized without optical resources. The sera were previously absorbed with heterologous NCTC strains and with the pellet of the sterile broth.
葡萄球菌协同凝集法被用作一种可由非专业实验室进行的快速鉴定支原体的方法。用针对NCTC支原体菌株的兔抗体致敏的金黄色葡萄球菌（考恩I型）悬液已鉴定出这些微生物，以及从人、细胞培养物、大鼠和小鼠中分离出的菌株，这些菌株来自4.0毫升培养物的浓缩悬液。通过葡萄球菌协同凝集法鉴定了48株肺炎支原体、6株关节炎支原体、8株精氨酸支原体、3株口腔支原体、15株莱氏无胆甾原体、8株人型支原体和3株肺炎支原体，并通过生长抑制试验进行了确认。确定了协同凝集的最佳参数，当加入乙酰半胱氨酸时，结合物的稳定性可保持90天。该反应无需光学设备即可显现。血清预先用异源NCTC菌株和无菌肉汤的沉淀进行了吸收。
