Baringer J R, Pisani P
Department of Neurology, Veterans Administration Medical Center, Salt Lake City, UT 84148.
Ann Neurol. 1994 Dec;36(6):823-9. doi: 10.1002/ana.410360605.
Herpes simplex virus (HSV) is known to establish latency in human trigeminal ganglia. It has been speculated that the virus might also be present in latent fashion in normal human brain, where it might be responsible for conditions such as herpes simplex encephalitis, and less plausibly as a cause for multiple sclerosis or Alzheimer's disease. To test the possibility that HSV exists in normal human brain, we utilized the polymerase chain reaction to assess the frequency and distribution of HSV genomes in the nervous system tissues of patients dying of nonneurological causes. Nine samples were obtained in a systematic fashion from olfactory bulb, gyrus rectus, hippocampus amygdala, calcarine cortex, pons, medulla, cerebellum, and trigeminal ganglia from each of 40 individuals dying of nonneurological disease. HSV genomes were sought in each sample using primers from four regions of the HSV genome. The primers were capable of detecting HSV genomic sequences from as little as 10 fg of DNA. HSV genomic sequences were identified in 26 (65%) of 40 samples of trigeminal ganglia. From 30 patients seropositive to HSV, sequences were amplified from 23 (77%). HSV genomic sequences could be amplified and detected in 14 (35%) of 40 brains. The positive areas included medulla, olfactory bulbs, pons, gyrus rectus, amygdala, and hippocampus. The study has confirmed the previous demonstration of latent HSV in trigeminal ganglia in normal humans. The frequency of latent HSV in trigeminal ganglia is in general agreement with results obtained by explanation of ganglia.(ABSTRACT TRUNCATED AT 250 WORDS)
已知单纯疱疹病毒（HSV）可在人类三叉神经节中建立潜伏感染。据推测，该病毒在正常人类大脑中也可能以潜伏形式存在，在那里它可能与单纯疱疹性脑炎等病症有关，而作为多发性硬化症或阿尔茨海默病的病因则不太可信。为了检测HSV是否存在于正常人类大脑中，我们利用聚合酶链反应来评估死于非神经疾病患者神经系统组织中HSV基因组的频率和分布。从40名死于非神经疾病的个体中，以系统方式从嗅球、直回、海马杏仁核、距状皮质、脑桥、延髓、小脑和三叉神经节获取了9个样本。使用来自HSV基因组四个区域的引物在每个样本中寻找HSV基因组。这些引物能够检测低至10 fg DNA中的HSV基因组序列。在40个三叉神经节样本中的26个（65%）中鉴定出HSV基因组序列。在30名HSV血清阳性患者中，从23个样本（77%）中扩增出序列。在40个大脑中的14个（35%）中可以扩增并检测到HSV基因组序列。阳性区域包括延髓、嗅球、脑桥、直回、杏仁核和海马。该研究证实了先前关于正常人类三叉神经节中潜伏HSV的证明。三叉神经节中潜伏HSV的频率与通过神经节切片获得的结果总体一致。（摘要截断于250字）
