由[14C]乙酸盐和14CO2进行丙二酰辅酶A的半制备酶促合成：1、2或3位标记
A semi-preparative enzymic synthesis of malonyl-CoA from [14C]acetate and 14CO2: labelling in the 1, 2 or 3 position.
作者信息
Roughan G
机构信息
Horticulture and Food Research Institute of New Zealand Ltd., Mt. Albert Research Centre, Auckland.
出版信息
Biochem J. 1994 Jun 1;300 ( Pt 2)(Pt 2):355-8. doi: 10.1042/bj3000355.
Abstract
A semi-preparative enzymic synthesis of [1-14C]malonyl-CoA from [1-14C]acetate and bicarbonate, and of [3-14C]malonyl-CoA from Na2(14)CO3 and acetate, was achieved by using chloroplasts rapidly isolated from 7-8-day-old pea shoots. Around 70% of the [1-14C]acetate was converted into malonyl-CoA in 2-3 h, and the specific radioactivity of [3-14C]malonyl-CoA synthesized in the system was 25-30 Ci/mol. Reactions were monitored and labelled products were purified by h.p.l.c.
摘要
利用从7 - 8日龄豌豆嫩梢快速分离的叶绿体，实现了从[1-¹⁴C]乙酸盐和碳酸氢盐半制备酶法合成[1-¹⁴C]丙二酰辅酶A，以及从Na₂(¹⁴)CO₃和乙酸盐半制备酶法合成[3-¹⁴C]丙二酰辅酶A。在2 - 3小时内，约70%的[1-¹⁴C]乙酸盐转化为丙二酰辅酶A，该系统中合成的[3-¹⁴C]丙二酰辅酶A的比放射性为25 - 30 Ci/mol。通过高效液相色谱法监测反应并纯化标记产物。
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