Galey D, Destrade C, Jaffard R
Laboratoire de Neurosciences Comportementales et Cognitives, CNRS URA 339, Université de Bordeaux I, Talence, France.
Behav Brain Res. 1994 Feb 28;60(2):183-9. doi: 10.1016/0166-4328(94)90146-5.
This experiment was designed to highlight the relationships between septo-hippocampal cholinergic activation and the processing of memory consolidation. For that purpose, we have analyzed the consequences of a medial septal electrical stimulation (100 Hz, 30 microA) applied soon after partial acquisition session of an appetitive operant conditioning task on in vivo hippocampal cholinergic activity on the one hand and on subsequent retention 24 h later on the other hand. For maximize our data base for such comparison we used two neurochemically and behaviorally distincts strains of mice, BALB/c and C57BL/6. In these conditions, our results showed that BALB/c mice evidenced better performance in retention than C57BL/6 mice after medial septal stimulation. On the other hand, the stimulation applied in resting conditions produced a moderate and similar hippocampal cholinergic activation in the two strains. Moreover, in BALB/c mice the only strain which exhibited good consolidation capacities the stimulation do not induced any additive effect on the substantial increase of the hippocampal cholinergic activity produced by the previous acquisition session. Finally, a correlative study realized in C57BL/6 mice seems to indicate that the higher the hippocampal cholinergic activity was the lower were the consolidation capacities. These results lead us to suggest that the improvement of memory consolidation induced by the medial septal stimulation is the consequence of the recruitment of non cholinergic elements located close to the electrode tip. Consequently the septo-hippocampal cholinergic activation is more likely to facilitate certain information processes prior to the consolidation mechanisms proper.
本实验旨在突出隔海马胆碱能激活与记忆巩固过程之间的关系。为此，我们分析了在一项食欲性操作性条件反射任务的部分习得训练后不久施加内侧隔区电刺激（100赫兹，30微安）对体内海马胆碱能活性的影响，以及24小时后对后续记忆保持的影响。为了最大限度地增加用于此类比较的数据库，我们使用了两种神经化学和行为上不同的小鼠品系，BALB/c和C57BL/6。在这些条件下，我们的结果表明，内侧隔区刺激后，BALB/c小鼠在记忆保持方面的表现优于C57BL/6小鼠。另一方面，在静息条件下施加的刺激在两个品系中产生了适度且相似的海马胆碱能激活。此外，在BALB/c小鼠（唯一表现出良好巩固能力的品系）中，该刺激并未对先前习得训练所产生的海马胆碱能活性的大幅增加产生任何累加效应。最后，在C57BL/6小鼠中进行的一项相关性研究似乎表明，海马胆碱能活性越高，巩固能力越低。这些结果使我们提出，内侧隔区刺激所诱导的记忆巩固改善是靠近电极尖端的非胆碱能成分被募集的结果。因此，隔海马胆碱能激活更有可能在适当的巩固机制之前促进某些信息处理过程。
