Astles R, Williams C P, Sedor F
Department of Pathology, Duke University Medical Center, Durham, NC 27710.
Clin Chem. 1994 Jul;40(7 Pt 1):1327-30.
The use of plasma lactate to assess metabolic or circulatory impairment requires definition of critical preanalytical and analytical parameters. Stability has been documented for only 15 min after acquisition when samples were collected with fluoride and transported on ice. We examined time elapsed before analysis, storage temperature, and the antiglycolytic agent used to define preanalytical conditions. Plasma lactate was measured with a Kodak Ektachem 700XR analyzer. In controlled studies on volunteers, storage on ice slowed but did not eliminate the production of lactate; for samples collected with sodium fluoride (F) and potassium oxalate (OX), lactate increased by 0.2 mmol/L after 1 h, then changed little regardless of the storage temperature. For patients' samples collected in F/OX, the mean increase was only 0.15 mmol/L after 24 h. Samples with leukocytosis (neutrophil counts 23 x 10(9)-52 x 10(9)/L) were also stable, with a mean increase of 0.3 mmol/L at 8 h. Use of the antiglycolytic agents F and OX (at 60 and 12 mmol/L, respectively) maintained apparently stable lactate concentrations at room temperature for up to 8 h without special handling.
使用血浆乳酸来评估代谢或循环功能损害需要明确关键的分析前和分析参数。当样本用氟化物采集并在冰上运输时，采集后仅15分钟内乳酸水平具有稳定性记录。我们研究了分析前的时间间隔、储存温度以及用于确定分析前条件的抗糖酵解剂。使用柯达Ektachem 700XR分析仪测量血浆乳酸。在针对志愿者的对照研究中，冰上储存减缓了但并未消除乳酸的产生；对于用氟化钠（F）和草酸钾（OX）采集的样本，1小时后乳酸增加了0.2 mmol/L，之后无论储存温度如何变化都很小。对于用F/OX采集的患者样本，24小时后平均增加仅为0.15 mmol/L。白细胞增多症患者（中性粒细胞计数为23×10⁹ - 52×10⁹/L）的样本也具有稳定性，8小时时平均增加0.3 mmol/L。使用抗糖酵解剂F和OX（分别为60和12 mmol/L）在室温下无需特殊处理即可使乳酸浓度在长达8小时内保持明显稳定。
