Dartois V, Coppée J Y, Colson C, Baulard A
Laboratoire de Génétique Microbienne, Université Catholique de Louvain, Belgium.
Appl Environ Microbiol. 1994 May;60(5):1670-3. doi: 10.1128/aem.60.5.1670-1673.1994.
The previously cloned Bacillus subtilis lipase gene (lip) was mapped on the chromosome and used in the construction of a B. subtilis derivative totally devoid of any lip sequence. Homologous overexpression was performed in this strain by subcloning the lip open reading frame on a multicopy plasmid under the control of a strong gram-positive promoter. A 100-fold overproducing strain was obtained, which should facilitate purification of the secreted protein. Furthermore, the delta lip strain BCL1050 constitutes an ideal host for the cloning of heterologous lipase genes.
先前克隆的枯草芽孢杆菌脂肪酶基因（lip）被定位到染色体上，并用于构建完全不含任何lip序列的枯草芽孢杆菌衍生物。通过将lip开放阅读框亚克隆到多拷贝质粒上，在强革兰氏阳性启动子的控制下，在该菌株中进行同源过表达。获得了一个产量提高100倍的菌株，这将有助于分泌蛋白的纯化。此外，lip缺失菌株BCL1050构成了克隆异源脂肪酶基因的理想宿主。
