Guidry G
Neural Development Section, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, Maryland 20892, USA.
J Histochem Cytochem. 1999 Feb;47(2):261-4. doi: 10.1177/002215549904700215.
A method is described for counterstaining tissue for use with the glyoxylic acid reaction for visual detection of biogenic amines. Counterstaining is achieved by addition of the fluorescent dye malachite green to the sucrose-phosphate-glyoxylic acid (SPG) solution used for processing of cryostat sections of unfixed tissues. When bound to tissues, the dye provides red-orange fluorescence of background tissue, which contrasts well with the green to yellow fluorescence induced by the glyoxylic acid reaction product formed with biogenic amines. The counterstaining technique is demonstrated in a number of catecholamine-containing peripheral tissues and is compared to sections that were processed without counterstaining.
本文描述了一种用于对组织进行复染的方法，该方法用于与乙醛酸反应配合，以视觉检测生物胺。通过将荧光染料孔雀石绿添加到用于处理未固定组织的低温恒温器切片的蔗糖 - 磷酸盐 - 乙醛酸（SPG）溶液中来实现复染。当染料与组织结合时，它会使背景组织发出红橙色荧光，这与由生物胺形成的乙醛酸反应产物诱导的绿色到黄色荧光形成良好对比。在一些含儿茶酚胺的外周组织中展示了这种复染技术，并与未进行复染处理的切片进行了比较。
