Khan Q A, Hadi S M
Department of Biochemistry, Faculty of Life Sciences, Aligarh Muslim University, India.
Biochem Mol Biol Int. 1994 Feb;32(2):379-85.
Furfural is a dietary mutagen and is present in various frequently consumed food products. In earlier work we have shown that it induces single strand breaks in duplex DNA which occur preferentially in AT base pairs. Experiments on the conservation of EcoRI cleavage site in mutant plasmids further established that repair of furfural damaged plasmid DNA occurs on propagation in the host cells. In this paper it is shown that the strand scissions induced by furfural in DNA account for its biological activity as assayed by inactivation of bacteriophage lambda. Using various repair defective mutants of E. coli we have shown that DNA repair predominantly involves the polA pathway.
糠醛是一种饮食诱变剂，存在于各种常见的食用产品中。在早期的研究中，我们已经表明它会诱导双链DNA中的单链断裂，这种断裂优先发生在AT碱基对中。对突变体质粒中EcoRI切割位点保守性的实验进一步证实，糠醛损伤的质粒DNA在宿主细胞中繁殖时会发生修复。本文表明，糠醛在DNA中诱导的链断裂解释了其通过λ噬菌体失活测定的生物活性。利用大肠杆菌的各种修复缺陷突变体，我们已经表明DNA修复主要涉及polA途径。
