Trophilme C, Becker A M, Jaffard H P, Jaulin P
Secteur d'hémobiologie-transfusion de Paris-Nord.
Transfus Clin Biol. 1995;2(3):189-99. doi: 10.1016/s1246-7820(05)80046-1.
The results of irregular antibody screening in two successive series of 21485 and 19819 individuals were retrospectively analysed. All the irregular antibody screenings were performed with an autoanalyser according to Rosenfield and Lalezary [1, 2] but red cell antibody identification tests were not the same: conventional manual tests in the first series and gel-test Diamed in the second one. In spite of a bigger number of identification tests performed in the second series, the percentage of patients with irregular antibodies was approximately the same (2.27% and 2.22%) in each series. Analysis of identified specificities shows in the second series the increase of immune antibodies (+ 42.3%) especially antibodies with anti-Rh specificity and the decline of natural antibodies (- 28.9%) essentially of anti-Lewis b and P1 antibodies. The sensitivity of both the gel-test and the autoanalyser screening for identification of immune antibodies are more or less the same. The sensitivity of gel-test probably gives a safer transfusion but increases the workload of both the laboratory and the blood bank delivery service.
对连续两组分别为21485人和19819人的不规则抗体筛查结果进行了回顾性分析。所有不规则抗体筛查均使用自动分析仪按照罗森菲尔德和拉莱扎里的方法进行[1,2]，但红细胞抗体鉴定试验不同：第一组采用传统手工试验，第二组采用DiaMed凝胶试验。尽管第二组进行的鉴定试验数量更多，但每组中不规则抗体患者的百分比大致相同（分别为2.27%和2.22%）。对鉴定出的特异性进行分析表明，在第二组中免疫抗体增加（+42.3%），尤其是具有抗Rh特异性的抗体，而天然抗体减少（-28.9%），主要是抗Lewis b和P1抗体。凝胶试验和自动分析仪筛查对免疫抗体鉴定的敏感性大致相同。凝胶试验的敏感性可能使输血更安全，但增加了实验室和血库供血服务的工作量。
