在永井高胆红素血症大鼠中进行肝细胞移植后的肝胆转运

Hepatic biliary transport after hepatocyte transplantation in Eizai hyperbilirubinemic rats.

作者信息

Hamaguchi H, Yamaguchi Y, Goto M, Misumi M, Hisama N, Miyanari N, Mori K, Ogawa M

机构信息

Department of Surgery II, Kumamoto University Medical School, Japan.

出版信息

Hepatology. 1994 Jul;20(1 Pt 1):220-4. doi: 10.1016/0270-9139(94)90156-2.

DOI:10.1016/0270-9139(94)90156-2

PMID:8020892

Abstract

The Eizai hyperbilirubinemic rat (EHBR) is a Sprague-Dawley mutant rat with conjugated hyperbilirubinemia. Eizai hyperbilirubinemic rats have impaired canalicular excretory transport of organic anions, bile acid glucuronide and sulfate. Eizai hyperbilirubinemic rats, with and without a 68% partial hepatectomy, were treated by an intraportal injection of 1 x 10(7) wild-type Sprague-Dawley mutant rat hepatocytes. Serum bilirubin concentrations decreased significantly after hepatectomy and intraportal injection. Biliary excretion was demonstrated by biliary transport of indocyanine and sulfobromophthalein into the bile. These results indicate that hepatic transport of bile acid conjugates in Eizai hyperbilirubinemic rats can be restored by hepatocyte transplantation in conjunction with the recipient's excretory biliary system.

摘要

荣研高胆红素血症大鼠（EHBR）是一种患有结合型高胆红素血症的斯普拉格-道利突变大鼠。荣研高胆红素血症大鼠的胆小管有机阴离子、胆汁酸葡糖醛酸和硫酸盐的排泄转运受损。对有和没有进行68%部分肝切除术的荣研高胆红素血症大鼠通过门静脉内注射1×10⁷野生型斯普拉格-道利突变大鼠肝细胞进行治疗。肝切除和门静脉内注射后血清胆红素浓度显著降低。通过将吲哚菁绿和磺溴酞钠转运至胆汁中证明了胆汁排泄。这些结果表明，荣研高胆红素血症大鼠胆汁酸结合物的肝脏转运可通过肝细胞移植结合受体的排泄胆管系统得以恢复。