Pietschmann P, Cush J J, Lipsky P E, Oppenheimer-Marks N
Harold C. Simmons Arthritis Research Center, University of Texas Southwestern Medical Center, Dallas 75235.
J Immunol. 1992 Aug 15;149(4):1170-8.
A critical step in immunologically mediated inflammation is the migration of T cells between endothelial cells of postcapillary venules and into the tissues. To determine whether specific cells are capable of transendothelial migration, T cells that had migrated through endothelial monolayers were retrieved and analyzed. To accomplish this, human umbilical vein endothelial cells (EC) were cultured to confluence on collagen gels and incubated with human T cells. T cells that were nonadherent to the EC, those that bound to the endothelium, and cells that had migrated through the endothelial monolayer and into the collagen were individually harvested and characterized. After a 4-h incubation with EC, T cells distributed themselves such that 77 +/- 2% were nonadherent, 13 +/- 2% were bound to EC, and 10 +/- 1% had migrated into the collagen. The CD4+ T cells that had migrated into the collagen were predominantly CD29bright/CD45RObright and CD45RA-. CD8+ T cells demonstrated a greater transendothelial migratory capacity than the CD4+ T cells. The migrated CD8+ T cells were mainly CD29bright but CD45RA+. Additional phenotypic analysis of the migrating cells indicated that they contained fewer cells that expressed L-selectin. Moreover the surface expression of CD7 was less dense in the T cells that had migrated than in the nonadherent T cells. Finally the T cells that migrated were not enriched for CD45RBdim T cells. Prolonging the incubation with EC to 36 h increased the number of T cells that migrated but did not alter the predominance of CD29bright T cells in the migrated population. Stimulation of EC with IL-1 or IFN-gamma also increased the number of adherent and migrating T cells, respectively, but did not alter the phenotype of the migrating cells. These results indicate that the capacity for transendothelial migration is an intrinsic ability of certain subpopulations of T cells and is related to their stage of differentiation as identified by their surface phenotype.
免疫介导炎症中的一个关键步骤是T细胞在毛细血管后微静脉的内皮细胞之间迁移并进入组织。为了确定特定细胞是否能够进行跨内皮迁移，对穿过内皮单层迁移的T细胞进行了回收和分析。为实现这一点，将人脐静脉内皮细胞（EC）培养至在胶原凝胶上汇合，并与人T细胞一起孵育。分别收获并鉴定未黏附于EC的T细胞、黏附于内皮的T细胞以及穿过内皮单层并进入胶原的细胞。与EC孵育4小时后，T细胞的分布情况如下：77±2%未黏附，13±2%黏附于EC，10±1%已迁移至胶原中。迁移至胶原中的CD4⁺T细胞主要为CD29亮/CD45RO亮和CD45RA⁻。CD8⁺T细胞显示出比CD4⁺T细胞更强的跨内皮迁移能力。迁移的CD8⁺T细胞主要为CD29亮，但为CD45RA⁺。对迁移细胞的进一步表型分析表明，表达L-选择素的细胞较少。此外，迁移的T细胞中CD7的表面表达密度低于未黏附的T细胞。最后，迁移的T细胞中CD45RB暗的T细胞并未富集。将与EC的孵育时间延长至36小时增加了迁移的T细胞数量，但并未改变迁移群体中CD29亮T细胞的优势地位。用IL-1或IFN-γ刺激EC分别增加了黏附的和迁移的T细胞数量，但并未改变迁移细胞的表型。这些结果表明，跨内皮迁移能力是某些T细胞亚群的内在能力，并且与其通过表面表型鉴定的分化阶段相关。
