Nishimori I, Yamamoto Y, Okazaki K, Morita M, Onodera M, Kino J, Tamura S, Yamamoto Y
First Department of Internal Medicine, Kochi Medical School, Japan.
Pancreas. 1994 May;9(3):374-81. doi: 10.1097/00006676-199405000-00015.
A serum autoantibody to a pancreatic antigen was identified in patients with idiopathic chronic pancreatitis and Sjögren's syndrome by radioimmunoassay and Western immunoblotting. Antigen from porcine and human pancreas extracts was partially purified using a monoclonal antibody, SP3-1, which recognizes the antigen in duct cells of various exocrine organs. Solid phase radioimmunoassay of the pancreatic antigen showed a positive result in 6 of 20 patients with idiopathic chronic pancreatitis (30%), 3 of 11 patients with Sjögren's syndrome (27%), and 1 of 15 patients with alcoholic chronic pancreatitis (7%). Among seven patients with stone-related chronic pancreatitis, six patients with autoimmune thyroiditis, and 14 normal controls, none showed evidence of autoantibodies to the pancreatic antigen. Western immunoblotting showed that serum antibody commonly reacted with a 60-kD molecule of either porcine or human pancreatic antigen, with which SP3-1 also reacted. These results show the existence of the autoantibodies to pancreas, especially to an antigen expressed in ductal cells of exocrine glands, in idiopathic chronic pancreatitis and Sjögren's syndrome, and suggest the possibility of an autoimmune mechanism in the pathogenesis of idiopathic chronic pancreatitis.
通过放射免疫分析和蛋白质免疫印迹法，在特发性慢性胰腺炎和干燥综合征患者中鉴定出一种针对胰腺抗原的血清自身抗体。使用单克隆抗体SP3-1对猪胰腺提取物和人胰腺提取物中的抗原进行部分纯化，该单克隆抗体可识别各种外分泌器官导管细胞中的抗原。胰腺抗原的固相放射免疫分析显示，20例特发性慢性胰腺炎患者中有6例（30%）、11例干燥综合征患者中有3例（27%）、15例酒精性慢性胰腺炎患者中有1例（7%）呈阳性结果。在7例结石相关性慢性胰腺炎患者、6例自身免疫性甲状腺炎患者和14名正常对照中，均未显示出针对胰腺抗原的自身抗体证据。蛋白质免疫印迹法显示，血清抗体通常与猪或人胰腺抗原的60-kD分子发生反应，SP3-1也与该分子发生反应。这些结果表明，在特发性慢性胰腺炎和干燥综合征中存在针对胰腺的自身抗体，尤其是针对外分泌腺导管细胞中表达的一种抗原的自身抗体，并提示特发性慢性胰腺炎发病机制中存在自身免疫机制的可能性。
