Snowden K C, Gardner R C
Centre for Gene Technology, School of Biological Sciences, University of Auckland, New Zealand.
Plant Physiol. 1993 Nov;103(3):855-61. doi: 10.1104/pp.103.3.855.
Five different cDNAs (termed wali1 to wali5 for Wheat Aluminum Induced) whose expression was induced by Al stress have been isolated from the root tips of Al-treated wheat (Triticum aestivum L.) plants. Four of these genes were induced 24 to 96 h after Al treatment, and their expression is reduced when the Al is removed. Each of these four genes was induced by inhibitory levels of Al in two wheat cultivars--Warigal, an Al-sensitive cultivar, and Waalt, an Al-tolerant cultivar. The fifth gene (wali2) showed a complex bimodal pattern of induction and was induced by Al only in the sensitive cultivar. Comparison of the nucleotide sequences of these clones to those in the sequence data bases showed that wali4 is homologous to phenylalanine ammonia-lyase and wali1 is homologous to a group of plant proteins that are cysteine-rich and have homology to metallothioneins. wali2 encodes a novel protein with a repeating motif of cysteine amino acids. The remaining two wali clones (wali3 and wali5) encode related, cysteine-rich proteins that show no significant homology to any known sequences.
从经铝处理的小麦（Triticum aestivum L.）植株根尖中分离出了5种不同的cDNA（因受小麦铝诱导而命名为wali1至wali5），其表达受铝胁迫诱导。其中4个基因在铝处理后24至96小时被诱导，去除铝后其表达降低。在两个小麦品种——铝敏感品种Warigal和铝耐受品种Waalt中，这4个基因中的每一个都被抑制水平的铝所诱导。第五个基因（wali2）表现出复杂的双峰诱导模式，且仅在敏感品种中被铝诱导。将这些克隆的核苷酸序列与序列数据库中的序列进行比较，结果表明wali4与苯丙氨酸解氨酶同源，wali1与一组富含半胱氨酸且与金属硫蛋白具有同源性的植物蛋白同源。wali2编码一种具有半胱氨酸氨基酸重复基序的新蛋白质。其余两个wali克隆（wali3和wali5）编码相关的、富含半胱氨酸的蛋白质，它们与任何已知序列均无明显同源性。
