Galelli M, Díaz Gómez M I, Castro J A
Centro de Investigaciones Toxicológicas (CEITOX)-CITEFA/CONICET, Buenos Aires, Argentina.
Arch Toxicol. 1994;68(3):206-9. doi: 10.1007/s002040050056.
Administration of CCl4 to 12-14 h starved Sprague Dawley male rats (220-240 g) at a dose of 5 ml of a 20% (v/v) olive oil solution/kg i.p. decreased the incorporation of 14C-leucine into microsomal and nuclear proteins at 1 or 2 h after the hepatotoxin. Decreased amino acid incorporation capacity in nuclear but not in microsomal proteins returned to control values at 4 h after poisoning. Cycloheximide given i.p. at a dose of 1 mg/kg in saline either alone or before CCl4 also decreased to a similar or greater extent the incorporation of 14C-leucine into both, microsomal and nuclear proteins at 1 h. The CCl4-induced decrease in amino acid incorporation was observable and significant even 1 h after CCl4 and involved histone and different non-histone nuclear protein fractions. Results suggest that decreased protein synthesis at the endoplasmic reticulum occurring in the very early stages of CCl4 poisoning might provoke temporary deficiencies in protein supplies to nuclei with as yet unknown consequences. However, some of the proteins involved have major regulatory properties in DNA expression.
给禁食12 - 14小时的斯普拉格-道利雄性大鼠（220 - 240克）腹腔注射5毫升/千克的20%（体积/体积）橄榄油溶液中的四氯化碳，在给予肝毒素后1或2小时，可降低14C - 亮氨酸掺入微粒体和核蛋白的量。中毒后4小时，核蛋白中氨基酸掺入能力降低，而微粒体蛋白中氨基酸掺入能力未降低，核蛋白中氨基酸掺入能力恢复到对照值。单独或以1毫克/千克的剂量在生理盐水中腹腔注射环己酰亚胺，无论是在四氯化碳之前还是之后，在1小时时也会使14C - 亮氨酸掺入微粒体和核蛋白的量降低到相似或更大程度。四氯化碳诱导的氨基酸掺入减少在四氯化碳注射后1小时就可观察到且很显著，涉及组蛋白和不同的非组蛋白核蛋白组分。结果表明，在四氯化碳中毒的早期阶段，内质网中蛋白质合成减少可能会引发细胞核蛋白质供应的暂时不足，其后果尚不清楚。然而，一些相关蛋白质在DNA表达中具有主要的调节特性。
