Dougherty M J, Hamilton J G, Ward R D
Division of Biomedical Sciences, Liverpool School of Tropical Medicine, U.K.
Med Vet Entomol. 1994 Apr;8(2):119-24. doi: 10.1111/j.1365-2915.1994.tb00150.x.
Semiochemical components of eggs of the sandfly Lutzomyia longipalpis (Diptera: Psychodidae) were separated by high performance liquid chromatography. HPLC fractions were examined quantitatively and qualitatively by gas chromatography (GC). A bioassay was used to determine the oviposition attraction of gravid L. longipalpis to each of the fractions separately and a peak responsible for the semiochemical activity was identified. Gravid flies were placed in individual oviposition tubes to determine if the peak of interest was an oviposition stimulant. The active semiochemical fraction attracted gravid flies for oviposition. Furthermore, egg laying was enhanced: gravid flies exposed to the pheromone oviposited earlier and laid more eggs than control flies. GC analysis indicated that 1200 eggs (2 days old) gave a yield of 12.75 micrograms of active pheromone. This fraction had similar HPLC and GC retention times to caryophyllene oxide, suggesting comparable polarity and molecular weight.
利用高效液相色谱法对长须罗蛉（双翅目：毛蠓科）卵的化学生态成分进行了分离。通过气相色谱法（GC）对高效液相色谱馏分进行了定量和定性分析。采用生物测定法分别测定妊娠长须罗蛉对各馏分的产卵吸引力，并鉴定了负责化学生态活性的一个峰。将妊娠蝇置于单独的产卵管中，以确定感兴趣的峰是否为产卵刺激物。活性化学生态馏分吸引妊娠蝇产卵。此外，产卵量增加：暴露于该信息素的妊娠蝇比对照蝇更早产卵且产卵更多。气相色谱分析表明，1200枚（2日龄）卵产生了12.75微克活性信息素。该馏分在高效液相色谱和气相色谱中的保留时间与氧化石竹烯相似，表明其极性和分子量相当。
