Pitt W G, McBride M O, Barton A J, Sagers R D
Chemical Engineering Department, Brigham Young University, Provo, UT 84602.
Biomaterials. 1993 Jul;14(8):605-8. doi: 10.1016/0142-9612(93)90179-6.
Video microscopy was employed to observed the spatial distribution of Staphylococcus epidermidis and Pseudomonas aeruginosa adherent to glass and polymer substrates. During rinsing procedures the bacteria remained in their original positions when the surfaces were rinsed with saline for 3 min followed by ethanol for 3 min before exposure to air. When the surfaces were rinsed with saline only, the air-liquid interface disrupted the spatial distribution of the bacteria, removing and redepositing the bacteria in clumps. A moving air-liquid interface of a gas bubble on substrate also displaced bacteria. Such artefacts produced by air-water interfaces should be avoided during bacterial adhesion experiments.
采用视频显微镜观察表皮葡萄球菌和铜绿假单胞菌在玻璃和聚合物基质上的空间分布。在冲洗过程中，当用盐水冲洗表面3分钟，然后在暴露于空气之前用乙醇冲洗3分钟时，细菌仍留在其原始位置。当仅用盐水冲洗表面时，气液界面破坏了细菌的空间分布，使细菌成团脱落并重新沉积。基质上气泡的移动气液界面也会使细菌移位。在细菌黏附实验中应避免气水界面产生的此类假象。
