Goodwin B L, Ruthven C R, Sandler M
Department of Chemical Pathology, Queen Charlotte's and Chelsea Hospital, London, U.K.
Biochem Pharmacol. 1994 Jun 15;47(12):2294-7. doi: 10.1016/0006-2952(94)90268-2.
The concentration of several phenolic acids and alcohols was measured in urine from germ-free and specific pathogen-free (SPF) rats before and after inoculation with faecal microorganisms, and from conventional rats before and after gut sterilization. The rate of excretion of benzoic acid, phenylacetic acid, and m- and p-hydroxyphenylpropionic acid in the germ-free animals was markedly increased after inoculation. Some acids showed no increase, including the endogenously generated homovanillic, vanilmandelic and p-hydroxyphenyllactic acids. Most others sought showed a small but significant increase. Some of the compounds excreted by the germ-free animals may have been in the food pellets, either as such or as precursors. The pattern was somewhat different in the SPF rats. The excretion of p-hydroxyphenylpropionic, p-hydroxyphenylacetic and m-hydroxyphenylacetic acids was initially much higher than in the germ-free animals and their excretion decreased after inoculation, presumably because of an altered pattern of gut flora. This work quantifies the effect of gut flora in the formation of some of the more important phenolic acids found in rat urine.
在无菌大鼠和无特定病原体（SPF）大鼠接种粪便微生物前后，以及普通大鼠肠道灭菌前后，测量了其尿液中几种酚酸和醇的浓度。接种后，无菌动物体内苯甲酸、苯乙酸、间羟基苯丙酸和对羟基苯丙酸的排泄率显著增加。一些酸没有增加，包括内源性生成的高香草酸、香草扁桃酸和对羟基苯乳酸。其他大多数检测的酸显示出小幅但显著的增加。无菌动物排泄的一些化合物可能存在于食物颗粒中，要么原样存在，要么以前体形式存在。SPF大鼠的情况略有不同。对羟基苯丙酸、对羟基苯乙酸和间羟基苯乙酸的排泄最初比无菌动物高得多，接种后其排泄减少，可能是由于肠道菌群模式的改变。这项工作量化了肠道菌群在大鼠尿液中一些更重要酚酸形成中的作用。
