Bazylak G
Hygienics Department, Medical University of Lodz, Poland.
J Chromatogr A. 1994 May 13;668(2):519-27. doi: 10.1016/0021-9673(94)80148-7.
The use of a chiral mobile phase additive in the form of the helically distorted, square-planar, chiral nickel(II) chelate dl-[4,4'-(1-methyl-2-propylethane-1,2-diyldiimino)bis(pent-3 -en-2- onato)]nickel(II) was investigated for the resolution of optical isomers of dipeptide-type sweeteners, viz., aspartame, alitame and antiaspartame, and some of their decomposition products, e.g., diketopiperazines. The chiral discrimination mechanism for the solutes was elucidated. The proposed chiral RP-HPLC system was applied to the stereoselective determination of aspartame impurities in samples of its commercial dietetic and pharmaceutical formulations.
研究了以螺旋扭曲的平面正方形手性镍（II）螯合物dl-[4,4'-(1-甲基-2-丙基亚乙基二亚氨基)双(戊-3-烯-2-酮酸根)]镍（II）形式的手性流动相添加剂用于拆分二肽型甜味剂（即阿斯巴甜、阿力甜和反阿斯巴甜）及其一些分解产物（如二酮哌嗪）的光学异构体。阐明了溶质的手性识别机制。将所提出的手性反相高效液相色谱系统应用于其商业饮食和药物制剂样品中阿斯巴甜杂质的立体选择性测定。
