Zhang R, Jiang M
Central Laboratory, Zhejiang Sanitation and Antiepidemic Station, Hangzhou, 310009.
Se Pu. 1997 Nov;15(6):542-3.
A method for rapid determination of Aspartame in compound sweetening by reversed-phase high performance liquid chromatography is presented. Aspartame in compound sweetening was separated in a short column (Ultrasphere XL-ODS, 3 microm, 4.6 mm x 70 mm) by using CH3OH-0.02 mol/L NH4Ac as mobile phase. The flow rate was 0.8 mL/min. Detection was performed with UV detector at 220 nm. The injection volume was 20 microL. It was qualitatively analysed by UV scanning at a wavelength range of 200-350 nm under no-stop flow according to their retention time. Quantitative analysis was carried out by measuring peak height and comparing it with external standard. The minimum detectable amount was 5 microg/L. The linear range of the calibration curve was 40-200 mg/L. The average recovery of Aspartame was 92%. The relative standard deviation was 2.9%. This method is simple, rapid and sensitive.
介绍了一种用反相高效液相色谱法快速测定复合甜味剂中天冬甜素的方法。采用甲醇 - 0.02mol/L 醋酸铵作为流动相，在短柱（Ultrasphere XL - ODS，3 微米，4.6 毫米×70 毫米）中分离复合甜味剂中的天冬甜素。流速为 0.8 毫升/分钟。用紫外检测器在 220 纳米处进行检测。进样量为 20 微升。根据保留时间，在无停流情况下于 200 - 350 纳米波长范围内进行紫外扫描定性分析。通过测量峰高并与外标比较进行定量分析。最低检测量为 5 微克/升。校准曲线的线性范围为 40 - 200 毫克/升。天冬甜素的平均回收率为 92%。相对标准偏差为 2.9%。该方法简单、快速且灵敏。
