Polishing with T4 or Pfu polymerase increases the efficiency of cloning of PCR fragments.
作者信息
Costa G L, Weiner M P
机构信息
Stratagene Cloning Systems, La Jolla, CA 92037.
出版信息
Nucleic Acids Res. 1994 Jun 25;22(12):2423. doi: 10.1093/nar/22.12.2423.
Abstract
摘要
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2
Novel blunt-end addition reactions catalyzed by DNA polymerase I of Escherichia coli.大肠杆菌DNA聚合酶I催化的新型平端添加反应。
J Mol Biol. 1987 Nov 5;198(1):123-7. doi: 10.1016/0022-2836(87)90462-1.
3
Novel non-templated nucleotide addition reactions catalyzed by procaryotic and eucaryotic DNA polymerases.原核生物和真核生物DNA聚合酶催化的新型非模板核苷酸添加反应。
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Cloning of PCR products after defined cohesive termini are created with T4 DNA polymerase.用T4 DNA聚合酶产生特定粘性末端后对PCR产物进行克隆。
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