Varga J, Kevei F, Vágvölgyi C, Vriesema A, Croft J H
Department of Microbiology, Attila József University, Szeged, Hungary.
Can J Microbiol. 1994 Apr;40(4):325-9. doi: 10.1139/m94-054.
Double-stranded RNA bands were detected electrophoretically in about 7% of natural isolates and in 13 of 51 collection strains belonging in section Nigri of the genus Aspergillus. The identity of these bands was proved by S1 nuclease and RNase treatment. Most of the virus-containing natural isolates came from Indonesia. Electron microscopic examination of the strains revealed the presence of virus-like particles in the mycelia of the strains examined. All of the virus-like particles were isometric and their size was around 30-35 nm, while some Indonesian isolates also contained virus-like particles in the size range 23-25 nm. It was possible to cure some of these strains of virus-like particles by mutagenic treatment. The four strains tested lost their virus-like particles and also their 'arginine-proline leaky' phenotype and became prototrophic. Virus transfer was possible among these four strains by protoplast fusion. It also proved possible to transfer mycoviruses into a more distantly related Aspergillus tubingensis strain by prolonged incubation of the polyethylene glycol treated protoplasts in osmotically stabilized medium. In spite of the finding that all Aspergillus foetidus and both Aspergillus heteromorphus strains examined contained double-stranded RNA segments and virus-like particles, no phenotypes related to the presence of these VLPs have been observed so far.
在大约7%的自然分离株以及曲霉属黑曲霉组的51个收集菌株中的13个菌株中，通过电泳检测到了双链RNA条带。这些条带的身份通过S1核酸酶和核糖核酸酶处理得以证实。大多数含有病毒的自然分离株来自印度尼西亚。对这些菌株进行电子显微镜检查发现，在所检查的菌株菌丝体中存在病毒样颗粒。所有病毒样颗粒均为等轴状，大小约为30 - 35纳米，而一些印度尼西亚分离株还含有大小在23 - 25纳米范围内的病毒样颗粒。通过诱变处理有可能治愈其中一些含有病毒样颗粒的菌株。所测试的四个菌株失去了病毒样颗粒以及它们的“精氨酸 - 脯氨酸渗漏”表型，变成了原养型。通过原生质体融合可以在这四个菌株之间进行病毒转移。通过在渗透稳定培养基中长时间培养聚乙二醇处理过的原生质体，也证明有可能将真菌病毒转移到亲缘关系更远的泡盛曲霉菌株中。尽管发现所有检测的臭曲霉和异形曲霉菌株都含有双链RNA片段和病毒样颗粒，但迄今为止尚未观察到与这些病毒样颗粒存在相关的表型。
