Plant Biotechnology Research Group-Virology, Western Australian State Agricultural Biotechnology Centre, School of Veterinary and Life Sciences, Murdoch University, Perth 6150, Australia.
Viruses. 2019 Jan 21;11(1):89. doi: 10.3390/v11010089.
and are the most destructive fungal species infecting stone fruit ( species). High-throughput cDNA sequencing of and isolates collected from stone fruit orchards revealed that 14% of isolates were infected with one or more of three mycoviruses: Sclerotinia sclerotiorum hypovirus 2 (SsHV2, genus ), Fusarium poae virus 1 (FPV1, genus ), and Botrytis virus F (BVF, genus ). Isolate M196 of was co-infected with all three viruses, and this isolate was studied further. Several methods were applied to cure M196 of one or more mycoviruses. Of these treatments, hyphal tip culture either alone or in combination with antibiotic treatment generated isogenic lines free of one or more mycoviruses. When isogenic fungal lines were cultured on nutrient agar medium in vitro, the triple mycovirus-infected parent isolate M196 grew 10% faster than any of the virus-cured isogenic lines. BVF had a slight inhibitory effect on growth, and FPV1 did not influence growth. Surprisingly, after inoculation to fruits of sweet cherry, there were no significance differences in disease progression between isogenic lines, suggesting that these mycoviruses did not influence the virulence of on a natural host.
和 是感染核果（属）的最具破坏性的真菌物种。对来自核果园的 和 分离物进行高通量 cDNA 测序表明，14%的分离物感染了三种真菌病毒中的一种或多种：核盘菌潜隐病毒 2（SsHV2，属）、禾谷镰刀菌病毒 1（FPV1，属）和葡萄孢病毒 F（BVF，属）。分离物 M196 同时感染了这三种病毒，对其进行了进一步研究。应用了几种方法来治愈 M196 的一种或多种真菌病毒。在这些处理中，单独或与抗生素处理联合的菌丝尖端培养产生了一种或多种真菌病毒无感染的同基因系。当同基因真菌系在体外营养琼脂培养基上培养时，三重真菌病毒感染的亲本分离物 M196 的生长速度比任何一种病毒治愈的同基因系都快 10%。BVF 对生长有轻微的抑制作用，FPV1 不影响生长。令人惊讶的是，接种甜樱桃果实后，同基因系之间的病害进展没有显著差异，表明这些真菌病毒不会影响在天然宿主上的毒力。
