在不同患病率人群的生殖系统样本中，对Amplicor沙眼衣原体检测法与培养法进行评估。
Evaluation of the Amplicor Chlamydia trachomatis test versus culture in genital samples in various prevalence populations.
作者信息
de Barbeyrac B, Pellet I, Dutilh B, Bébéar C, Dumon B, Géniaux M, Bébéar C
机构信息
Laboratoire de Bactériologie, Hôpital Pellegrin, Bordeaux, France.
出版信息
Genitourin Med. 1994 Jun;70(3):162-6. doi: 10.1136/sti.70.3.162.
OBJECTIVE
To evaluate a newly developed polymerase chain reaction (PCR) assay, Amplicor C trachomatis for the detection of C trachomatis in genital samples using cell culture for comparison.
SUBJECTS
501 patients (431 women and 70 men) attending an STD clinic in Hôpital Pellegrin (high-risk population) and gynaecological clinics (low-risk population) in Bordeaux, France.
METHODS
The genital samples (cervical and urethral) were tested for the presence of C trachomatis using the Amplicor test and using standard cell culture identified by the immunofluorescence test using a monoclonal antibody to C trachomatis. Discrepancies between the results of culture and Amplicor were further analysed by major outer membrane protein gene (omp1)-PCR of the specimens taken in transport media and by direct fluorescent antibody (DFA) staining of elementary bodies in culture transport tubes.
RESULTS
After analysis of discrepancies, the revised sensitivity and specificity of PCR were 95.3% and 100% and the positive and negative predictive values were 100% and 99.5%, respectively.
CONCLUSION
The present results indicate that the Amplicor assay is rapid, specific and more sensitive than the culture method. This test provides an excellent non-culture method for the detection of C trachomatis in various prevalence populations.
目的
评估一种新开发的聚合酶链反应（PCR）检测方法——沙眼衣原体扩增检测法（Amplicor C trachomatis），通过与细胞培养法比较，用于检测生殖器样本中的沙眼衣原体。
研究对象
501名患者（431名女性和70名男性），来自法国波尔多市佩勒格林医院（高危人群）的性传播疾病诊所及妇科诊所（低危人群）。
方法
采用沙眼衣原体扩增检测法及标准细胞培养法检测生殖器样本（宫颈和尿道样本）中沙眼衣原体的存在情况，细胞培养法通过使用抗沙眼衣原体单克隆抗体的免疫荧光试验进行鉴定。对于培养结果与扩增检测法结果之间的差异，通过对运输培养基中采集的标本进行主要外膜蛋白基因（omp1）-PCR分析，以及对培养运输管中的原体进行直接荧光抗体（DFA）染色进一步分析。
结果
经过差异分析，PCR的校正敏感性和特异性分别为95.3%和100%，阳性预测值和阴性预测值分别为100%和99.5%。
结论
目前的结果表明，扩增检测法快速、特异，且比培养法更敏感。该检测方法为在不同流行率人群中检测沙眼衣原体提供了一种出色的非培养方法。
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