在泌尿生殖系统标本中，通过连接酶链反应检测沙眼衣原体，并与聚合酶链反应和细胞培养法进行比较。
Detection of Chlamydia trachomatis by ligase chain reaction compared with polymerase chain reaction and cell culture in urogenital specimens.
作者信息
de Barbeyrac B, Rodriguez P, Dutilh B, Le Roux P, Bébéar C
机构信息
Laboratoire de Bactériologie, Hôpital Pellegrin, Bordeaux, France.
出版信息
Genitourin Med. 1995 Dec;71(6):382-6. doi: 10.1136/sti.71.6.382.
OBJECTIVE
The aim of this study was to evaluate the newly developed ligase chain reaction (LCR) assay for the detection of Chlamydia trachomatis in urogenital specimens using cell culture and Amplicor PCR for comparison.
SUBJECTS
Two hundred and eighty patients attending hospital or urban STD clinics (high-risk population, 62 men and 84 women) and obstetric/gynaecology clinics (low-risk population, 134 women) in Bordeaux, France.
METHODS
Specimens from men were tested with LCR on urethral swabs and urine, with Amplicor or urine, with cell culture on urethral swabs. Specimens from women were tested with LCR, Amplicor and cell culture on endocervical swabs and with LCR on urine. When the three methods generated different results, the LCR and Amplicor tests were repeated on the remaining samples. Samples with discordant LCR and Amplicor results and a negative culture were further analysed by major outer membrane protein gene omp1-PCR.
RESULTS
After analysis of discrepant results, the overall prevalence was 7.5% (21/280) calculated on the basis of an expanded "gold standard" defined as culture positive or LCR plus Amplicor positive or omp1-PCR positive for discrepant results between LCR and Amplicor tests. Of the 21, 20 were detected by LCR, 17 by Amplicor and culture. The specificity of LCR and Amplicor was 99.6%.
CONCLUSION
The LCR Chlamydia trachomatis test is a highly sensitive nonculture technique and a good alternative test for the detection of chlamydial infections.
目的
本研究旨在评估新开发的连接酶链反应（LCR）检测法，用于检测泌尿生殖系统标本中的沙眼衣原体，并与细胞培养法和Amplicor聚合酶链反应（PCR）法进行比较。
对象
法国波尔多市280名前往医院或城市性传播疾病诊所（高危人群，62名男性和84名女性）以及妇产科诊所（低危人群，134名女性）就诊的患者。
方法
男性标本采用LCR检测尿道拭子和尿液，采用Amplicor检测尿道拭子或尿液，采用细胞培养法检测尿道拭子。女性标本采用LCR、Amplicor和细胞培养法检测宫颈拭子，并采用LCR检测尿液。当三种方法得出不同结果时，对剩余样本重复进行LCR和Amplicor检测。LCR和Amplicor结果不一致且培养结果为阴性的样本，进一步通过主要外膜蛋白基因omp1-PCR进行分析。
结果
在对不一致结果进行分析后，根据扩大的“金标准”计算得出总体患病率为7.5%（21/280），该“金标准”定义为培养阳性或LCR加Amplicor阳性或LCR与Amplicor检测结果不一致时omp1-PCR阳性。在这21例中，LCR检测出20例，Amplicor和培养法检测出17例。LCR和Amplicor的特异性为99.6%。
结论
LCR沙眼衣原体检测法是一种高度敏感的非培养技术，是检测衣原体感染的良好替代检测方法。
Zotero 插件