高效液相色谱法测定血浆中δ-氨基乙酰丙酸的研究

Study on measurement of delta-aminolevulinic acid in plasma by high-performance liquid chromatography.

作者信息

Miyajima K, Hirata M, Yoshida T, Kosaka H, Okayama A

机构信息

Department of Occupational Health, Osaka Prefectural Institute of Public Health, Japan.

出版信息

J Chromatogr B Biomed Appl. 1994 Apr 1;654(2):165-9. doi: 10.1016/0378-4347(94)00046-8.

DOI:10.1016/0378-4347(94)00046-8

PMID:8044276

Abstract

A method for the determination of delta-aminolevulinic acid in plasma of lead-exposed workers by high-performance liquid chromatography with fluorescence detection of a fluorescent delta-aminolevulinic acid derivative (2-methylidineamino-3,5-diacetyl-4,6-dimethylpropionic acid) was established. The detection limit of delta-aminolevulinic acid in plasma was 0.01 microgram/ml at a signal-to-noise ratio of 5:1. A linear correlation was obtained between the amounts of delta-aminolevulinic acid injected from 0.01 to 0.5 microgram/ml (r = 0.999). The recovery of 0.05 and 0.1 microgram/ml of delta-aminolevulinic acid added to plasma with various concentrations of delta-aminolevulinic acid in plasma ranged from 80.0 to 100.8%. This method, combined with the use of an automatic sampler, should facilitate the routine measurement of delta-aminolevulinic acid in plasma.

摘要

建立了一种用高效液相色谱法结合荧光检测荧光δ-氨基乙酰丙酸衍生物（2-亚甲基氨基-3,5-二乙酰基-4,6-二甲基丙酸）来测定铅接触工人血浆中δ-氨基乙酰丙酸的方法。在信噪比为5:1时，血浆中δ-氨基乙酰丙酸的检测限为0.01微克/毫升。注入的δ-氨基乙酰丙酸量在0.01至0.5微克/毫升之间呈线性相关（r = 0.999）。向含有不同浓度δ-氨基乙酰丙酸的血浆中添加0.05和0.1微克/毫升的δ-氨基乙酰丙酸，回收率在80.0%至100.8%之间。该方法结合自动进样器的使用，应有助于血浆中δ-氨基乙酰丙酸的常规检测。