Ostwald C, Müller P, Barten M, Rutsatz K, Sonnenburg M, Milde-Langosch K, Löning T
Institute of Pathology, University of Rostock, Germany.
J Oral Pathol Med. 1994 May;23(5):220-5. doi: 10.1111/j.1600-0714.1994.tb01117.x.
Human papillomavirus (HPV) infections in oral carcinomas and normal oral mucosa were studied by consensus primer screening and typing for HPV types 6/11, 16 and 18 DNA. After polymerase chain reaction (PCR) the DNA species of interest were identified by Southern blot hybridization with digoxigenin-labeled oligonucleotide probes. Frozen tissue and scrapings were equally suitable for HPV testing and yielded high HPV detection rates in carcinomas. By comparison, HPV analysis of paraffin-embedded material was much less efficient. HPV were demonstrated in 61.5% (16/26) of oral squamous cell carcinomas, high risk HPV 16 and 18 being the preferential types. The frequency of HPV detection in non-neoplastic mucosa of tumor patients decreased clearly with increasing distance from the tumor (range 26.9-3.8%) suggesting focal HPV infections. In contrast, normal buccal mucosa of a group of healthy volunteers contained HPV DNA only in 1% (1/97).
通过共识引物筛选以及对6/11、16和18型人乳头瘤病毒（HPV）DNA进行分型，研究了口腔癌和正常口腔黏膜中的HPV感染情况。聚合酶链反应（PCR）后，通过与地高辛标记的寡核苷酸探针进行Southern印迹杂交来鉴定感兴趣的DNA种类。冷冻组织和刮片同样适合HPV检测，并且在癌组织中HPV检测率很高。相比之下，石蜡包埋材料的HPV分析效率要低得多。在61.5%（16/26）的口腔鳞状细胞癌中检测到HPV，高危型HPV 16和18为优势类型。肿瘤患者非肿瘤性黏膜中HPV检测频率随着与肿瘤距离的增加而明显降低（范围为26.9 - 3.8%），提示HPV为局灶性感染。相比之下，一组健康志愿者的正常颊黏膜中仅1%（1/97）含有HPV DNA。
