杨树和拟南芥二氢吡啶二羧酸合酶cDNA克隆的分离
Isolation of a poplar and an Arabidopsis thaliana dihydrodipicolinate synthase cDNA clone.
作者信息
Vauterin M, Jacobs M
机构信息
Laboratorium voor Plantengenetica, Vrije Universiteit Brussel, St.-Genesius Rode, Belgium.
出版信息
Plant Mol Biol. 1994 Jun;25(3):545-50. doi: 10.1007/BF00043882.
PMID:8049377
Abstract
A poplar DHDPS cDNA clone has been isolated by functional rescue of the dapA-deficient AT997 mutant of Escherichia coli. By sequence comparison between the poplar and maize DHDPS cDNAs, two oligonucleotides were designed to perform polymerase chain reaction (PCR) on Arabidopsis thaliana genomic DNA. The PCR fragment was subsequently used to isolate an Arabidopsis DHDPS genomic and cDNA clone.
摘要
通过对大肠杆菌dapA缺陷型AT997突变体进行功能拯救，分离出了一个杨树DHDPS cDNA克隆。通过对杨树和玉米DHDPS cDNA进行序列比较，设计了两个寡核苷酸用于对拟南芥基因组DNA进行聚合酶链反应（PCR）。随后，该PCR片段被用于分离一个拟南芥DHDPS基因组和cDNA克隆。
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