Sarrobert C, Thibaud M C, Contard-David P, Gineste S, Bechtold N, Robaglia C, Nussaume L
Laboratoire du Métabolisme Carboné, UMR 163, CNRS-CEA, DSV.DEVM, CEA/Cadarache, F-13108 St Paul les Durance Cedex, France.
Plant J. 2000 Nov;24(3):357-67. doi: 10.1046/j.1365-313x.2000.00884.x.
A novel Arabidopsis DHDPS gene named DHDPS2 was found through identification of a mutant by promoter trapping. The mutation promotes a reduction of growth resulting from combination of a defect in lysine biosynthesis and accumulation of a toxic level of threonine or derived products. The mutant also modifies the amino acid composition issuing from the pyruvate and aspartate pathways, affecting mainly the root compartment. These data are in accordance with the expression of DHDPS2 in the root apex as visualized by expression of the GUS reporter gene. This suggests that a large proportion of the amino acids derived from pyruvate and aspartate are synthesized in this organ.
通过启动子捕获鉴定突变体，发现了一个名为DHDPS2的新型拟南芥DHDPS基因。该突变导致赖氨酸生物合成缺陷与有毒水平的苏氨酸或其衍生产物积累相结合，从而促进生长减少。该突变体还改变了丙酮酸和天冬氨酸途径产生的氨基酸组成，主要影响根部区域。这些数据与通过GUS报告基因表达可视化的根尖中DHDPS2的表达一致。这表明很大一部分源自丙酮酸和天冬氨酸的氨基酸是在该器官中合成的。
