Becker J C, Gillitzer R, Bröcker E B
Department of Dermatology, University of Würzburg, Germany.
Int J Cancer. 1994 Aug 1;58(3):346-8. doi: 10.1002/ijc.2910580306.
MAGE-1 has been identified as a human gene, which directs the expression of an antigen being recognized on melanoma cells by autologous cytolytic T cells. MAGE-1 is expressed in melanomas and some other tumors. It has been proposed that this gene may be linked to the transformation event and therefore might serve as an approach to precisely targeted immunotherapy. Prior to such an approach, extensive testing of normal human tissue is necessary to establish the tumor-specific nature of MAGE-1 expression. Similar to events that occur during neoplastic tumor growth and spreading, wound healing involves a complex interrelationship between various cell types which migrate, proliferate and differentiate. Therefore, we investigated the expression of MAGE-1 in skin during wound repair. We could detect MAGE-1 mRNA by RT-PCR followed by specific hybridization as well as by Northern blotting in human skin from the 1st to the 7th day after wounding. Comparison of the expression of MAGE mRNA with that of beta-actin mRNA showed that it is expressed in amounts equal to about and at least one-fifth that of beta-actin. Our data strongly suggest that MAGE mRNA expression is not necessarily linked to neoplastic transformation, but rather represents the function of a cellular gene which is activated during inflammation or early tissue repair.
MAGE-1已被鉴定为一种人类基因，它指导一种抗原的表达，这种抗原可被自体溶细胞性T细胞识别为黑色素瘤细胞上的抗原。MAGE-1在黑色素瘤和其他一些肿瘤中表达。有人提出，该基因可能与转化事件有关，因此可能成为精确靶向免疫治疗的一种方法。在采用这种方法之前，有必要对正常人体组织进行广泛测试，以确定MAGE-1表达的肿瘤特异性。与肿瘤生长和扩散过程中发生的事件类似，伤口愈合涉及各种细胞类型之间复杂的相互关系，这些细胞会迁移、增殖和分化。因此，我们研究了伤口修复过程中皮肤中MAGE-1的表达。在受伤后第1天至第7天的人体皮肤中，我们可以通过RT-PCR随后进行特异性杂交以及Northern印迹法检测到MAGE-1 mRNA。将MAGE mRNA的表达与β-肌动蛋白mRNA的表达进行比较，结果表明其表达量约为β-肌动蛋白的五分之一且至少为其五分之一。我们的数据强烈表明，MAGE mRNA的表达不一定与肿瘤转化相关，而是代表一种细胞基因在炎症或早期组织修复过程中被激活的功能。
