在表达人脊髓灰质炎病毒受体基因的重组鼠细胞系中脊髓灰质炎病毒的选择性分离
Selective isolation of poliovirus in recombinant murine cell line expressing the human poliovirus receptor gene.
作者信息
Hovi T, Stenvik M
机构信息
Enterovirus Laboratory, National Public Health Institute (KTL), Helsinki, Finland.
出版信息
J Clin Microbiol. 1994 May;32(5):1366-8. doi: 10.1128/jcm.32.5.1366-1368.1994.
Abstract
Sixty-eight laboratory strains representing 49 enterovirus, 10 adenovirus, and 3 reovirus serotypes were inoculated in a recombinant murine cell line expressing the human poliovirus receptor gene (L alpha cells). Only polioviruses caused cytopathic effect over a 10-day period. Likewise, only polioviruses were isolated, by use of L alpha cells, from 168 fecal specimens from children from developing countries. These results suggest that the recombinant L alpha cells can be used for selective isolation of poliovirus from clinical specimens.
摘要
将代表49种肠道病毒血清型、10种腺病毒血清型和3种呼肠孤病毒血清型的68个实验室菌株接种到表达人脊髓灰质炎病毒受体基因的重组鼠细胞系（Lα细胞）中。在10天的时间里，只有脊髓灰质炎病毒引起细胞病变效应。同样，利用Lα细胞，仅从发展中国家儿童的168份粪便标本中分离出脊髓灰质炎病毒。这些结果表明，重组Lα细胞可用于从临床标本中选择性分离脊髓灰质炎病毒。
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1
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2
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3
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Arch Virol. 1994;134(1-2):39-50. doi: 10.1007/BF01379105.
4
Detection of enteroviruses in groundwater with the polymerase chain reaction.用聚合酶链反应检测地下水中的肠道病毒。
Appl Environ Microbiol. 1993 May;59(5):1318-24. doi: 10.1128/aem.59.5.1318-1324.1993.
5
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J Clin Microbiol. 1993 Feb;31(2):395-9. doi: 10.1128/jcm.31.2.395-399.1993.
6
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7
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Lancet. 1986 Jun 21;1(8495):1427-32. doi: 10.1016/s0140-6736(86)91566-7.
8
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