Sambhi M P, Eggena P, Barrett J C, Tuck M, Wiedeman C E, Thananopavarn C
Circ Res. 1975 Jun;36(6 Suppl 1):28-37. doi: 10.1161/01.res.36.6.28.
The rate of angiotensin generation with added renin in plasma from patients with benign essential hypertension has been shown to be higher than in plasma from norm ensive controls. An index of the angiotensin generation rate in relation to to al plasma renin substrate (PRS-r index) has been defined which allows for screening for "activated" plasma. In hypertensive subjects, this index was shown to be higher than that of the normotensive subjects (61 plus or minus 2.4 SE, and 45 plus or minus 5 SE). The index did not correlate with the absolute levels of blood pressure, 24-hour sodium excretion, or plasma renin activity in hypertensive subjects either during the control period or during acute alterations of blood pressure, but was shown to respond in a parallel fashion with chronically induced changes in blood pressure and circulating levels of angiotensin I. By the use of an isolated system of human renin and homologous renin substrate, we have demonstrated that plasma from hypertensive subjects contains a modifier of the renin reaction which increases both V-max and Km of the system, behaving as an uncompetitive activator. No significant change was noted with the addition of normal plasma to the same isolated system.
在良性原发性高血压患者的血浆中，添加肾素后血管紧张素生成速率已被证明高于正常血压对照者的血浆。已定义了一个与总血浆肾素底物相关的血管紧张素生成速率指数（PRS-r指数），可用于筛查“活化”血浆。在高血压受试者中，该指数高于正常血压受试者（分别为61±2.4标准误和45±5标准误）。在对照期或血压急性改变期间，该指数与高血压受试者的血压绝对水平、24小时钠排泄量或血浆肾素活性均无相关性，但显示出与慢性诱导的血压变化和血管紧张素I循环水平呈平行反应。通过使用人肾素和同源肾素底物的离体系统，我们证明高血压受试者的血浆含有肾素反应的一种调节剂，它增加了该系统的V- max和Km，表现为非竞争性激活剂。向同一离体系统中添加正常血浆未观察到显著变化。
