Eggena P, Hidaka H, Barrett J D, Sambhi M P
J Clin Invest. 1978 Aug;62(2):367-72. doi: 10.1172/JCI109137.
The objective of this investigation was to determine whether heterogeneity of plasma renin substrate could be observed in states of steroid excess and various forms of hypertensive disease. In states of stimulated renin substrate production by estrogens or glucocorticoids, multiple forms of renin substrate were apparent when stimulation was excessive. Stimulation of substrate production caused by uremia associated with hypertension showed similar results. None, or only trace quantities of the additional forms of renin substrate were evident in subjects with normal or suppressed levels of plasma renin substrate. The additional forms of renin substrate could be distinguished from the normal form on the basis of cross-reactivity with a specific antiserum to the normal form, electrophoretic mobility, and kinetic rate constants. Differences in rate constants of the various forms of plasma renin substrate may account for the altered rate of the renin reaction associated with several states of hypertension. In plasma of patients with renovascular hypertension, significant quantities of a protein which cross-reacted with the antiserum but could not generate angiotensin I were observed.
本研究的目的是确定在类固醇过量状态和各种形式的高血压疾病中是否能观察到血浆肾素底物的异质性。在雌激素或糖皮质激素刺激肾素底物产生的状态下，当刺激过度时，多种形式的肾素底物很明显。与高血压相关的尿毒症引起的底物产生刺激显示出类似的结果。在血浆肾素底物水平正常或受抑制的受试者中，没有或仅有微量的额外形式的肾素底物明显。额外形式的肾素底物可以根据与正常形式的特异性抗血清的交叉反应性、电泳迁移率和动力学速率常数与正常形式区分开来。各种形式的血浆肾素底物的速率常数差异可能解释了与几种高血压状态相关的肾素反应速率的改变。在肾血管性高血压患者的血浆中，观察到大量与抗血清发生交叉反应但不能产生血管紧张素I的蛋白质。
