Kolesnik Iu M, Orestenko Iu N, Abramov A V
Probl Endokrinol (Mosk). 1993 Jan-Feb;39(1):45-8.
The endocrine status of the pancreas and the hypothalamic neurosecretory nuclei were studied by radioimmunoassay, immunocytochemical, morphometric and histochemical methods in Wistar rats of both sexes with experimental diabetes mellitus. The development of diabetes mellitus was characterized by beta-cell destruction and insulin concentrations reduction in these cells and the blood, by increase of glucagon and somatostatin levels in the alpha- and delta-cells, respectively, as well as by the growth of these substances concentrations in the peripheral blood. These changes were parallelled by activation of the vasopressin-, oxytocin and corticoliberin-synthesizing neurones of the paraventricular and supraoptic nuclei of the hypothalamus, as evidenced by morphometric findings and by increase of the blood vasopressin and corticoliberin concentrations and oxytocin level in the hypothalamus. Experimental diabetes mellitus was found to be characterized by activation of the hypothalamo-hypophyseo-adrenal system. Functional differences in the contribution of vasopressin- and oxytocin-synthesizing neurones of the hypothalamic nuclei in the pathogenesis of the disease is shown, as are their sex-specific reactions.
采用放射免疫分析、免疫细胞化学、形态计量学和组织化学方法，对患有实验性糖尿病的雌雄Wistar大鼠的胰腺内分泌状态和下丘脑神经分泌核进行了研究。糖尿病的发展特征为β细胞破坏、这些细胞和血液中胰岛素浓度降低，α细胞和δ细胞中胰高血糖素和生长抑素水平分别升高，以及外周血中这些物质浓度增加。形态计量学结果以及下丘脑血液中血管加压素、促肾上腺皮质激素释放素浓度升高和催产素水平升高证明，这些变化与下丘脑室旁核和视上核中合成血管加压素、催产素和促肾上腺皮质激素释放素的神经元激活同时发生。实验性糖尿病的特征是下丘脑-垂体-肾上腺系统激活。研究显示了下丘脑核中合成血管加压素和催产素的神经元在疾病发病机制中的功能差异，以及它们的性别特异性反应。
